Fn1tm1Feb
Targeted Allele Detail
|
|
| Symbol: |
Fn1tm1Feb |
| Name: |
fibronectin 1; targeted mutation 1, Francisco E Baralle |
| MGI ID: |
MGI:2655527 |
| Synonyms: |
EDAfl |
| Gene: |
Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
|
| Alliance: |
Fn1tm1Feb page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:84274
|
| Parent Cell Line: |
Not Specified (ES Cell)
|
| Strain of Origin: |
129X1/SvJ
|
|
| Allele Type: |
|
Targeted (Conditional ready, Modified isoform(s)) |
| Mutation: |
|
Nucleotide substitutions
|
| |
|
Mutation details: The endogenous EDA exon, which is subject to alternative splicing, was replaced with a floxed EDA exon modified with point mutations for optimization of both the 5' and the 3' splice sites. RNase protection and RT-PCR analysis confirmed constitutive EDA exon inclusion, showing the EDA exon to be included in 99-100% of transcript in all tissues of homozygous mutant mice.
(J:84274)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
| Original: |
J:84274 Muro AF, et al., Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan. J Cell Biol. 2003 Jul 7;162(1):149-60 |
| All: |
10 reference(s) |
|
Analysis Tools
