Rbp3 RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:16741 Liou GI, et al., Timing of interphotoreceptor retinoid-binding protein (IRBP) gene expression and hypomethylation in developing mouse retina. Dev Biol. 1994 Feb;161(2):345-56
Assay type:	RNase protection
MGI Accession ID:	MGI:2655805
Gene symbol:	Rbp3
Gene name:	retinol binding protein 3, interstitial

Probe:	IRBP cDNA1
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography
Assay notes:	Authors give expression as a percentage of percent of adult expression this, is shown in a graph in Fig. 2.B. A point for E13.5, 80% of adult expression, is shown on the graph but not shown in the autoradiograph in Figure 12. A. It is included as lane 18. Given values are estimated from the graph in Fig. 2.B.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	245 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
E11	E11.5 (a)	TS19: retina	Trace (b)	40 µg; total RNA	C57BL/6		Not Specified
E12	E12.5 (a)	TS20: retina	Trace (b)	40 µg; total RNA	C57BL/6		Not Specified
E13	E13.5 (a)	TS21: retina	Present (c)	15 µg; total RNA	C57BL/6		Not Specified
E14	E14.5 (a)	TS22: retina	Present (d)	15 µg; total RNA	C57BL/6		Not Specified
E15	E15.5 (a)	TS23: retina	Present (e)	15 µg; total RNA	C57BL/6		Not Specified
E16	E16.5 (a)	TS24: retina	Present (f)	20 µg; total RNA	C57BL/6		Not Specified
E17	E17.5 (a)	TS25: retina	Present (g)	20 µg; total RNA	C57BL/6		Not Specified
E18	E18.5 (a)	TS26: retina	Present (h)	20 µg; total RNA	C57BL/6		Not Specified
Br	E11.5 (a)	TS19: brain	Absent	Not Specified; total RNA	C57BL/6		Not Specified
E19	E19.5 (a)	TS26: retina	Present (i)	20 µg; total RNA	C57BL/6		Not Specified
P0	P newborn (a)	TS27: retina	Present (j)	10 µg; total RNA	C57BL/6		Not Specified
P3	P3 (a)	TS27: retina	Present (k)	10 µg; total RNA	C57BL/6		Not Specified
P6	P6 (a)	TS28: retina	Present (l)	10 µg; total RNA	C57BL/6		Not Specified
P10	P10 (a)	TS28: retina	Present (m)	10 µg; total RNA	C57BL/6		Not Specified
P16	P16 (a)	TS28: retina	Present (n)	10 µg; total RNA	C57BL/6		Not Specified
P20	P20 (a)	TS28: retina	Present (o)	10 µg; total RNA	C57BL/6		Not Specified
P27	P27 (a)	TS28: retina	Present (p)	10 µg; total RNA	C57BL/6		Not Specified
P13	P13	TS28: retina	Present (n)	10 µg; total RNA	C57BL/6		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression is 0.2% of wild type.
(c) Expression is 0.9% of wild type.
(d) Expression is 2.2% of wild type.
(e) Expression is 1.7% of wild type.
(f) Expression is 2.3% of wild type.
(g) Expression is 3.3% of wild type.
(h) Expression is 3.4% of wild type.
(i) Expression is 8% of wild type.
(j) Expression is 7% of wild type.
(k) Expression is 25% of wild type.
(l) Expression is 40% of wild type.
(m) Expression is 65% of wild type.
(n) Expression is 80% of wild type.
(o) Expression is 90% of wild type.
(p) Expression is 100% of wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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