Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:22646
Tsumaki N, et al., Differential expression of an acidic domain in the amino-terminal propeptide of mouse pro-alpha 2(XI) collagen by complex alternative splicing. J Biol Chem. 1995 Feb 3;270(5):2372-8
|
Assay type:
|
RT-PCR
|
MGI Accession ID:
|
MGI:2656794
|
Gene symbol:
|
Col11a2
|
Gene name:
|
collagen, type XI, alpha 2
|
Probe:
|
Primer A, Primer B
|
Assay notes:
|
The RT-PCR reaction, which amplified the cDNA region corresponding to exons 5-10 was electrophoresed (see Figure 5B) and Southern blotted with an ECL 3'-oligolabeling and detection system. The probe was from exon 7 sequence (MGI:2656769).
|
|
Results
|
Mol. Wt. Marker Lane |
P w 3
|
TS28: cartilage
|
Absent
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: skeletal muscle
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: brain
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: heart
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: eye
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: skeletal system
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: liver
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
Mol. Wt. Marker Lane |
E10.0
(c)
|
TS16: forelimb bud
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
E11.0
(c)
|
TS18: forelimb bud
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
E12.0
(c)
|
TS20: forelimb
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
E14.0
(c)
|
TS22: forelimb
|
Not Specified
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
P w 3
|
TS28: cartilage
|
Absent
|
Not Specified
|
5 µg; total RNA |
Not Specified |
| Not Specified |
Mol. Wt. Marker Lane |
Notes:
|