Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:50953
Jiang S, et al., Strain-dependent developmental relaxation of imprinting of an endogenous mouse gene, Kvlqt1. Genomics. 1998 Nov 1;53(3):395-9
|
|
Assay type:
|
RT-PCR
|
|
MGI Accession ID:
|
MGI:2659116
|
|
Gene symbol:
|
Kcnq1
|
|
Gene name:
|
potassium voltage-gated channel, subfamily Q, member 1
|
|
Probe:
|
U1, L1
|
|
Assay notes:
|
After RT-PCR analyses, the bands were extracted and digested with BsmFI which cleaves the CAST/Ei allele into a 140 and 97 bp fragments. This assay showed that this gene is imprinted in all tissues tested except brain which has significant relaxation of imprinting which was confimred by a Southern blot shown in Fig. 3b.
|
|
|
Results
|
Image: 3a
| Control |
| Control |
| P adult
|
TS28: mouse
|
Present
|
Absent
|
1 µg; total RNA |
129S/SvEv |
| Female |
Specific tissues were not detailed.
|
| P adult
|
TS28: mouse
|
Absent
|
Present
|
1 µg; total RNA |
CAST/EiJ |
| Male |
Specific tissues were not detailed.
|
| P adult
|
TS28: heart
|
Present
|
Absent
|
1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
|
| P adult
|
TS28: liver
|
Present
|
Absent
|
1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
|
| P adult
|
TS28: brain
|
Present
|
Present
|
1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
|
| P adult
|
TS28: skeletal muscle
|
Present
|
Absent
|
1 µg; total RNA |
involves: 129S/SvEv * CAST/Ei |
| Not Specified |
|
Notes:
|
Analysis Tools
