Mt1tm1Rch
Targeted Allele Detail
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| Symbol: |
Mt1tm1Rch |
| Name: |
metallothionein 1; targeted mutation 1, Anna E Michalska |
| MGI ID: |
MGI:2668454 |
| Synonyms: |
MT-I -, MT-null |
| Gene: |
Mt1 Location: Chr8:94905710-94906955 bp, + strand Genetic Position: Chr8, 46.34 cM
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| Alliance: |
Mt1tm1Rch page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:14416
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| Parent Cell Line: |
E14 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutation: |
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Insertion
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Mutation details: Both metallothionein I and II were disrupted using the same targeting construct. Exon 1 of metallothionein I was disrupted by insertion of a 20 bp synthetic oligomer just downstream from the start codon which caused a frame shift. Northern blot analysis of liver mRNA from homozygotes in which the gene was induced by intraperitoneal injection of copper indicated that transcription did take place in spite of the frame shift. However, translation of the protein product did not occur.
(J:14416)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:14416 Michalska AE, et al., Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8088-92 |
| All: |
26 reference(s) |
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Analysis Tools
