Mug1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:28325 Overbergh L, et al., Expression of mouse alpha-macroglobulins, lipoprotein receptor-related protein, LDL receptor, apolipoprotein E, and lipoprotein lipase in pregnancy. J Lipid Res. 1995 Aug;36(8):1774-86
Assay type:	Northern blot
MGI Accession ID:	MGI:2671051
Gene symbol:	Mug1
Gene name:	murinoglobulin 1

Probe:	MUG cDNA2
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	A graphical representation of this data by densitometric analysis is shown in Figure 3B.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	5 kb (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
4	P adult	TS28: liver	Present	10 µg; total RNA	C57BL		Female	pregnant mouse at E3.5.
7	P adult	TS28: liver	Present	10 µg; total RNA	C57BL		Female	pregnant mouse at E6.5.
10	P adult	TS28: liver	Weak	10 µg; total RNA	C57BL		Female	pregnant mouse at E9.5.
13	P adult	TS28: liver	Weak	10 µg; total RNA	C57BL		Female	pregnant mouse at E12.5.
16	P adult	TS28: liver	Weak	10 µg; total RNA	C57BL		Female	pregnant mouse at E15.5.
19	P adult	TS28: liver	Present	10 µg; total RNA	C57BL		Female	pregnant mouse at E18.5.
+1	P adult	TS28: liver	Present	10 µg; total RNA	C57BL		Female	lactating mouse at postpartum day 1.
+7	P adult	TS28: liver	Weak	10 µg; total RNA	C57BL		Female	lactating mouse at postpartum day 7.
C	P adult	TS28: liver	Present	10 µg; total RNA	C57BL		Female	non-pregnant mouse.

Notes:
(a) Expression levels decreased 3 fold over the course of pregnancy. Lowest levels (30% of non-pregnant control) was reached between days 9.5 and 15.5 of pregnancy. Levels were normal at birth, but decreased again at P7 to 60% of non-pregnant control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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