Dnase2btm1Osa
Targeted Allele Detail
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| Symbol: |
Dnase2btm1Osa |
| Name: |
deoxyribonuclease II beta; targeted mutation 1, Shigekazu Nagata |
| MGI ID: |
MGI:2675502 |
| Synonyms: |
DLAD- |
| Gene: |
Dnase2b Location: Chr3:146286740-146321351 bp, - strand Genetic Position: Chr3, 72.0 cM
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| Alliance: |
Dnase2btm1Osa page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:85215
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. Included in the deleted region were codons for 2 conserved histidine residues that are putative enzymatic active sites. Truncated transcript lacking the targeted sequence, and consequently containing a frameshift mutation, was detected in mutant mice by both Northern and RT-PCR analyses. Absence of DNAse activity under acidic conditions indicated that stable and functional protein was not produced by the mutant transcript.
(J:85215)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:85215 Nishimoto S, et al., Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature. 2003 Aug 28;424(6952):1071-4 |
| All: |
2 reference(s) |
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Analysis Tools
