Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79391
Fujiwara T, et al., Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse. Development. 2002 Oct;129(20):4685-96
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2676363
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Gene symbol:
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Vcam1
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Gene name:
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vascular cell adhesion molecule 1
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Antibody:
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anti-VCAM1 antibody
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
3A:
embryonic day 8.5
(more  )
Note: 9 somite stage.
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive ventricle |
Present |
Regionally restricted |
3A
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Authors report expression in the myocardium mostly in the primitive ventricle.
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Specimen
3D:
(close )
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Genetic Background:
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involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Bmp4tm1Blh/Bmp4tm1Blh
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Age:
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embryonic day 8.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive ventricle |
Weak |
Regionally restricted |
3D
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Expression in the myocardium mostly in the primitive ventricle; levels are reduced compared with wild-type.
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Specimen
4G:
embryonic day 8.5
(more )
Note: 9 somite stage.
Specimen
4G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: heart |
Present |
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4G
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Authors report normal rightward looping of heart tube.
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Analysis Tools