Top1tm1Unc
Targeted Allele Detail
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| Symbol: |
Top1tm1Unc |
| Name: |
topoisomerase (DNA) I; targeted mutation 1, University of North Carolina |
| MGI ID: |
MGI:2678314 |
| Synonyms: |
Top-1 - |
| Gene: |
Top1 Location: Chr2:160487901-160564684 bp, + strand Genetic Position: Chr2, 80.96 cM, cytoband H2-3
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| Alliance: |
Top1tm1Unc page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:36546
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| Parent Cell Line: |
BK4 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A neomycin selection cassette replaced a genomic fragment containing exon 15. Western blot analysis demonstrated that a 45% reduction of encoded protein levels was present in cells derived from heterozygous mice. Cytological analysis of early homozygous mutant embryos demonstrated that no detectable protein was produced from this allele.
(J:36546)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Top1 Mutation: |
210 strains or lines available
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| Original: |
J:36546 Morham SG, et al., Targeted disruption of the mouse topoisomerase I gene by camptothecin selection. Mol Cell Biol. 1996 Dec;16(12):6804-9 |
| All: |
1 reference(s) |
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