Tg(SLC18A3-cre)AMisa
Transgene Detail
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| Symbol: |
Tg(SLC18A3-cre)AMisa |
| Name: |
transgene insertion A, Hidemi Misawa |
| MGI ID: |
MGI:2679331 |
| Synonyms: |
VAChT-Cre-A, VACht.Cre.Slow |
| Transgene: |
Tg(SLC18A3-cre)AMisa Location: unknown
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| Alliance: |
Tg(SLC18A3-cre)AMisa page
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| Transgene Type: |
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Transgenic (Recombinase) |
| Mutation: |
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Insertion
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Tg(SLC18A3-cre)AMisa expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Homolog in Mouse |
Note |
| human |
SLC18A3 (6572) |
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Mutation details: The transgenic construct consisted of an 11.3 kb fragment of the human cholinergic gene locus, containing the first 5 exons of CHAT and the first coding exon of SLC18A3 (located in intron 1 of CHAT). A cassette containing cre followed by IRES-EGFP was inserted at the start codon of the SLC18A3 gene. EGFP expression was undetected. The 11.3 kb fragment included various promoter elements including a distal enhancer, RE1/NRSE, and NGFRE. Founder mice contained approximately 50 copies. Expression of cre recombinase by the human SLC18A3 promoter began at postnatal day 7 and was restricted to a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus. Maximal levels of expression were reached around 8 months ofage, later onset than in the Tg(SLC18a3-cre)KMisa line. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons.
(J:86160)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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SLC18A3
(human)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:86160 Misawa H, et al., VAChT-Cre.Fast and VAChT-Cre.Slow: postnatal expression of Cre recombinase in somatomotor neurons with different onset. Genesis. 2003 Sep;37(1):44-50 |
| All: |
3 reference(s) |
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Analysis Tools
