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Gene Expression Data
Assay Details
Assay
Reference: J:48293 Reule M, et al., Analysis of Peg1/Mest imprinting in the mouse. Dev Genes Evol. 1998 May;208(3):161-3
Assay type: RT-PCR
MGI Accession ID: MGI:2681479
Gene symbol: Mest
Gene name: mesoderm specific transcript
Probe: Mest-pA, Mest-pB
Assay notes: The RT-PCR sample was digested with the restriction endonuclease TaqI prior to electrophoresis. The mus allele gives three bands of 284, 173 and 30bp. The spretus allele gives two bands of 284 and 203bp.
Results Image: 1C
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Size Not Specified (b) Amount Genetic BackgroundMutant Allele(s)Sex Note
marker 2 Mol. Wt. Marker Lane
yolk sac E17.5 (c) TS25: yolk sac Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
placenta E17.5 (c) TS28: placenta Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Female Only the paternally inherited mus allele was detected.
skin E17.5 (c) TS25: skin Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
skeletal muscle E17.5 (c) TS25: skeletal muscle Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
duodenum E17.5 (c) TS25: duodenum Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
colon E17.5 (c) TS25: colon Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
liver E17.5 (c) TS25: liver Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
lung E17.5 (c) TS25: lung Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
heart E17.5 (c) TS25: heart Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
adipose tissue E17.5 (c) TS25: trunk mesenchyme Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
brain E17.5 (c) TS25: brain Absent Present Not Specified; total RNA involves: C3H * C57BL/6 * M. spretus Not Specified Only the paternally inherited mus allele was detected.
Notes:
(a) This band is present in the spretus allele.
(b) This band is present in the mus allele.
(c) Age normalized so that noon of plug day = E0.5.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory