Rorctm2Litt
Targeted Allele Detail
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| Symbol: |
Rorctm2Litt |
| Name: |
RAR-related orphan receptor gamma; targeted mutation 2, Dan R Littman |
| MGI ID: |
MGI:3026635 |
| Synonyms: |
Roc(gt)gfp, Rorc(gammat)-, Rorc(gammat)-EGFP, RorcGFP, RORgammat, RORgammat-EGFP |
| Gene: |
Rorc Location: Chr3:94280106-94305583 bp, + strand Genetic Position: Chr3, 40.56 cM, cytoband F2
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| Alliance: |
Rorctm2Litt page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:87395
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| Parent Cell Line: |
E14 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Insertion
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Mutation details: An isoform-specific disruption was generated by inserting a cassette containing eGFP downstream of the start codon of exon 1gammat. The targeted exon is the third exon of the gene, serves as an alternative first exon for the gammat transcript, and is not included in the gamma transcript. RT-PCR analysis of hepatic RNA obtained from homozygous mutant mice identified gamma transcript but not the targted gammat transcript. The eGFP sequence included a stop codon but no splice sites or polyadenylation signals. A floxed neo cassette included in the targeting vector was excised from the germline via cre-mediated recombination.
(J:87395)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:87395 Eberl G, et al., An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004 Jan;5(1):64-73 |
| All: |
182 reference(s) |
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Analysis Tools
