Shroom3 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:58691 Hildebrand JD, et al., Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell. 1999 Nov 24;99(5):485-97
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:3037557
Gene symbol:	Shroom3
Gene name:	shroom family member 3

Reporter:	lacZ
	Direct Detection

Results

Specimen 2A: embryonic day 8.5-8.75; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Strong	Regionally restricted	2A	Expression was detected in the cranial neuroepithelium in the rostral portion of the embryo.
TS13: gut	Present		2A
TS13: neural tube	Present		2A
TS13: trunk paraxial mesenchyme	Present		2A
TS13: head mesenchyme	Absent		2A

Specimen 2B left: embryonic day 10.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS18: forebrain	Present		2B left
TS18: gut	Present		2B left
TS18: heart	Present		2B left
TS18: neural tube	Present		2B left
TS18: trunk somite	Present		2B left
TS18: trunk	Present	Regionally restricted	2B left	Expression was detected in the ventral body wall.

Specimen 2B right: embryonic day 10.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS18: forebrain	Present		2B right
TS18: gut	Present		2B right
TS18: heart	Present		2B right
TS18: neural tube	Present		2B right
TS18: trunk somite	Present		2B right
TS18: trunk	Present	Regionally restricted	2B right	Expression was detected in the ventral body wall.

Specimen 2C: embryonic day 12.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		2C

Specimen 2D: embryonic day 14.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: forebrain	Present		2D
TS22: skeletal muscle	Present		2D
TS22: hand	Present	Regionally restricted	2D	Expression was detected in the distal tips of the digits.

Specimen 2E: embryonic day 8.5-8.75; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Strong	Regionally restricted	2E	Expression was detected in the cranial neuroepithelium in the rostral portion of the embryo.
TS13: gut	Present	Regionally restricted	2E	Expression was detected in the foregut.
TS13: neural tube	Present		2E
TS13: optic pit	Present		2E
TS13: trunk paraxial mesenchyme	Present		2E
TS13: head mesenchyme	Absent		2E

Specimen 2F: embryonic day 8.5-8.75; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		2F
TS13: trunk paraxial mesenchyme	Present		2F
TS13: gut	Present	Regionally restricted	2F	Expression was detected in the foregut.

Specimen 2G: embryonic day 8.5-8.75; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present		2G
TS13: trunk paraxial mesenchyme	Present		2G
TS13: gut	Present	Regionally restricted	2G	Expression was detected in the hindgut.

Specimen 2H: embryonic day 10.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		2H
TS17: gut	Present		2H
TS17: heart	Present		2H
TS17: neural tube	Present		2H
TS17: neural tube floor plate	Present		2H
TS17: neural tube roof plate	Present		2H
TS17: trunk somite	Present		2H
TS17: trunk	Present	Regionally restricted	2H	Expression was detected in the ventral body wall.

Specimen 2I: embryonic day 10.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: gut	Present		2I
TS17: heart	Present		2I
TS17: neural tube	Present		2I
TS17: neural tube floor plate	Present		2I
TS17: trunk somite	Present		2I
TS17: trunk	Present	Regionally restricted	2I	Expression was detected in the ventral body wall.

Specimen 2J: embryonic day 10.5; Shroom3^{Gt(ROSA53)Sor}/Shroom3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: heart	Present		2J
TS17: midgut	Present		2J
TS17: neural tube	Present		2J
TS17: neural tube floor plate	Present		2J
TS17: neural tube roof plate	Present		2J
TS17: trunk somite	Present		2J
TS17: trunk	Present	Regionally restricted	2J	Expression was detected in the ventral body wall.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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