Wt1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82515 Miles CG, et al., Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile. Mol Cell Biol. 2003 Apr;23(7):2608-13
Assay type:	Western blot
MGI Accession ID:	MGI:3038125
Gene symbol:	Wt1
Gene name:	WT1 transcription factor

Antibody:

6F-H2 (M3561)

Results

Image: 3B

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	54 kDa (b)	47 kDa (c)	Genetic Background	Mutant Allele(s)	Sex
+/+	E16.5 (a)	TS24: metanephros	Present	Present	involves: C57BL/6 * CD-1		Not Specified
-/-	E16.5 (a)	TS24: metanephros	Absent	Present	involves: 129P2/OlaHsd * C57BL/6 * CD-1	Wt1^tm1Hst/Wt1^tm1Hst	Not Specified
+/-	E16.5 (a)	TS24: metanephros	Present	Present	involves: 129P2/OlaHsd * C57BL/6 * CD-1	Wt1^tm1Hst/Wt1⁺	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) 54-56 kDa isoforms, labeled CUG, appear as a doublet corresponding to the presence or absence of the alternatively spliced exon 5.
(c) 47-49 kDa isoforms, labeled AUG, alternatively spliced for exon 5 comigrate on the gel.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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