Probe:	Pax6 probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		5A/B
TS12: future midbrain	Absent		5A/B

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		5C/D
TS12: future spinal cord neural fold	Absent		5C/D	No expression was seen in the neural groove.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present		5E/F
TS13: neural tube	Present		5E/F
TS13: future hindbrain	Present	Regionally restricted	5E/F	There is no expression in the presumptive metencephalon.
TS13: future midbrain	Absent		5E/F

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Single cells	5G/H	Expression is mainly restricted to mitotically active cells in the ventral ventricular zone.
TS17: hindbrain	Present		5G/H	Expression is present along the entire anterior-posterior axis up to the rhombencephalic isthmus.
TS17: diencephalon	Present		5G/H
TS17: nasal pit	Present		5G/H	Expression is present in the nasal pit.
TS17: midbrain	Absent		5G/H

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	6A/B	Expression is absent in the most dorsal and ventral cells, as well as the cells adjacent to the floor plate. Expression was not seen in the more caudal regions of the future spinal cord.
TS13: neural tube floor plate	Absent		6A/B
TS13: trunk somite	Absent		6A/B

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Graded	6C/D	There are high expression levels in the ventral portion of the neural tube; expression is weaker dorsally. Expression is not present in the cells immediately adjacent to the floor plate.
TS14: future spinal cord neural plate	Absent		6C/D
TS14: neural tube floor plate	Absent		6C/D
TS14: trunk somite	Absent		6C/D	There is no expression in the mesoderm adjacent to the neural tube.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	6E/F	Expression is present in the ventral part of the neural tube but has begun to regress dorsally. It is not present in cells immediately adjacent to the floor plate.
TS17: neural tube floor plate	Absent		6E/F

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present	Regionally restricted	6G/H	Expression is present in the ventral part of the neural tube but has begun to regress dorsally. It is not present in cells immediately adjacent to the floor plate.
TS19: neural tube mantle layer	Absent		6G/H	There is no expression in the intermediate zone.
TS19: neural tube floor plate	Absent		6G/H

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	6I/J	Expression is present in the ventral part of the neural tube but has begun to regress dorsally. It is not present in cells immediately adjacent to the floor plate.
TS20: spinal cord mantle layer	Present	Spotted	6I/J	There is spotted expression present in the intermediate zone.
TS20: spinal cord floor plate	Absent		6I/J

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present		6K/L	Expression is present in the ventral part of the neural tube but has begun to regress dorsally.
TS20: spinal cord mantle layer	Present	Spotted	6K/L	There is spotted expression present in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: spinal cord ventricular layer	Present	Regionally restricted	6M/N	Expression is present in the ventral part of the neural tube but has begun to regress dorsally. It is not present in cells immediately adjacent to the floor plate.
TS21: spinal cord mantle layer	Present	Spotted	6M/N	There is spotted expression present in the intermediate zone.
TS21: spinal cord floor plate	Absent		6M/N

Structure	Level	Pattern	Image	Note
TS23: spinal cord ventricular layer	Present	Regionally restricted	6O/P	Expression is present in a subset of ependymal cells around the wider part of the residual lumen of the neural tube but not in the ependymal layer lining the rest of the lumen. A low signal is observed ventrally in a subset of postmitotic cells.
TS23: spinal cord floor plate	Absent		6O/P

Structure	Level	Pattern	Image	Note
TS19: telencephalon ventricular layer	Strong		7A/B	High levels of transcript are detected in the lateral and dorsal neural epithelium but not in the basal telencephalon.
TS19: diencephalon	Present	Regionally restricted	7A/B	Expression is present up to a sharp caudal border at the posterior commissure. Expression is strong in the ventral thalamus, weak in the dorsal thalamus, and present in the hypothalamus and epithalamus.
TS19: hindbrain	Present		7A/B	Expression is present in the neural tube along the entire anterior-posterior axis up to the rhombencephalic isthmus.
TS19: myelencephalon	Present		7A/B	Expression is strongest in the ventricular zone.
TS19: metencephalon	Absent		7A/B
TS19: midbrain	Absent		7A/B
TS19: olfactory epithelium	Present		7A/B

Structure	Level	Pattern	Image	Note
TS20: telencephalon ventricular layer	Strong		7C/D	High levels of transcript are detected in the lateral and dorsal neural epithelium but not in the basal telencephalon.
TS20: diencephalon	Present	Regionally restricted	7C/D	Expression is present up to a sharp caudal border at the posterior commissure.
TS20: medulla oblongata	Present		7C/D	Expression is strongest in the ventricular zone.
TS20: epithalamus	Present		7C/D
TS20: hypothalamus	Present	Regionally restricted	7C/D	Expression is present in the anterior.
TS20: thalamus	Present	Graded	7C/D	There is strong expression present in the ventral thalamus; it is weaker in the dorsal thalamus.
TS20: metencephalon	Absent		7C/D
TS20: midbrain	Absent		7C/D
TS20: adenohypophysis pars anterior	Present		7C/D
TS20: adenohypophysis pars intermedia	Present		7C/D
TS20: remnant of Rathke's pouch	Present		7C/D
TS20: neurohypophysis infundibulum	Absent		7C/D
TS20: neurohypophysis pars nervosa	Absent		7C/D
TS20: olfactory epithelium	Present		7C/D

Structure	Level	Pattern	Image	Note
TS23: telencephalon ventricular layer	Strong		7E/F	High levels of transcript are detected in the lateral and dorsal neural epithelium but not in the basal telencephalon.
TS23: diencephalon	Present	Regionally restricted	7E/F	Expression is present up to a sharp caudal border at the posterior commissure.
TS23: medulla oblongata	Present		7E/F	Expression is strongest in the ventricular zone.
TS23: pineal primordium	Present		7E/F	Expression is present in the pineal gland.
TS23: epithalamus	Present	Regionally restricted	7E/F	Expression is present in the nuclei habenula.
TS23: zona incerta	Present		7E/F	Expression is present in the presumptive zona incerta.
TS23: olfactory lobe	Strong		7E/F
TS23: midbrain	Absent		7E/F
TS23: cerebellum	Present	Regionally restricted	7E/F	Expression is present in the external granule layer and the dorsal cerebellum.
TS23: pons	Present	Regionally restricted	7E/F	Expression is present in the pontine nuclei.
TS23: adenohypophysis pars anterior	Present		7E/F
TS23: adenohypophysis pars intermedia	Present		7E/F
TS23: remnant of Rathke's pouch	Present	Regionally restricted	7E/F	Expression is present in the epithelial lining of the lumen.
TS23: neurohypophysis infundibulum	Absent		7E/F
TS23: neurohypophysis pars nervosa	Absent		7E/F
TS23: nasal cavity epithelium	Present		7E/F

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	7G/H	High levels of transcript are detected in the ventricular layer of the lateral and dorsal neural epithelium but not in the basal telencephalon.
TS24: diencephalon	Present	Regionally restricted	7G/H	Expression is present up to a sharp caudal border at the posterior commissure.
TS24: medulla oblongata	Present		7G/H	Expression is strongest in the ventricular zone.
TS24: pons	Present	Regionally restricted	7G/H	Expression is present in the pontine nuclei.
TS24: cerebellum	Present	Regionally restricted	7G/H	Expression is present in the external granular layer.
TS24: zona incerta	Present		7G/H	Expression is present in the presumptive zona incerta.
TS24: olfactory lobe	Strong		7G/H
TS24: midbrain	Absent		7G/H
TS24: adenohypophysis pars anterior	Present		7G/H
TS24: adenohypophysis pars intermedia	Present		7G/H
TS24: remnant of Rathke's pouch	Present		7G/H
TS24: neurohypophysis infundibulum	Absent		7G/H
TS24: neurohypophysis pars nervosa	Absent		7G/H
TS24: olfactory epithelium	Present		7G/H

Structure	Level	Pattern	Image	Note
TS26: telencephalon	Strong	Regionally restricted	7I/J	High levels of transcript are detected in the lateral and dorsal neural epithelium but not in the basal telencephalon.
TS26: diencephalon	Present	Regionally restricted	7I/J	Expression is present up to a sharp caudal border at the posterior commissure.
TS26: epithalamus	Present	Regionally restricted	7I/J	Expression is present in the nuclei habenula.
TS26: medulla oblongata	Present		7I/J	Expression is strongest in the ventricular zone.
TS26: metencephalon	Present		7I/J
TS26: pineal gland	Present		7I/J
TS26: zona incerta	Present		7I/J	Expression is present in the presumptive zona incerta.
TS26: olfactory lobe	Strong		7I/J
TS26: midbrain	Absent		7I/J
TS26: cerebellum	Present		7I/J
TS26: pons	Present	Regionally restricted	7I/J	Expression is present in the pontine nuclei.
TS26: adenohypophysis pars anterior	Trace		7I/J	Expression levels are just above background.
TS26: adenohypophysis pars intermedia	Trace		7I/J	Expression levels are just above background.
TS26: remnant of Rathke's pouch	Trace		7I/J	Expression levels are just above background.
TS26: neurohypophysis infundibulum	Absent		7I/J
TS26: neurohypophysis pars nervosa	Absent		7I/J
TS26: olfactory epithelium	Present		7I/J

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present		7K/L
TS19: future adenohypophysis	Present		7K/L
TS19: myelencephalon	Present		7K/L	Expression is strongest in the ventricular zone.
TS19: future neurohypophysis	Absent		7K/L

Structure	Level	Pattern	Image	Note
TS12: optic sulcus	Present

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted		Expression is strong the ventral thalamus, weak in the dorsal thalamus, and present in the anterior of the hypothalamus.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Weak			Expression is in the granule layer.

Structure	Level	Pattern	Image	Note
TS14: optic vesicle	Present	Regionally restricted	8A/B	Expression is present in the epithelial layer of the vesicle and the optic stalk.
TS14: optic eminence surface ectoderm	Present	Regionally restricted	8A/B

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Present	Regionally restricted	8C/D	Expression is present in the epithelial layer of the vesicle and in the surface ectoderm.
TS15: optic stalk	Present		8C/D
TS15: lens placode	Present		8C/D

Structure	Level	Pattern	Image	Note
TS17: lens pit	Strong		8E/F
TS17: optic cup inner layer	Strong		8E/F
TS17: optic cup outer layer	Weak		8E/F

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong		8G/H
TS19: neural retinal epithelium	Strong		8G/H
TS19: pigmented retinal epithelium	Weak		8G/H
TS19: cornea	Present		8G/H

Structure	Level	Pattern	Image	Note
TS19: neural retinal epithelium	Strong		8I/J
TS19: pigmented retinal epithelium	Weak		8I/J
TS19: cornea	Present		8I/J
TS19: lens vesicle	Present		8I/J

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Strong		8K/L
TS20: pigmented retinal epithelium	Weak		8K/L
TS20: lens vesicle	Present	Regionally restricted	8K/L	Expression is present in the differentiating lens fiber cells in the posterior wall of the lens.
TS20: optic stalk	Present	Regionally restricted	8K/L	Expression is present in a narrow ventricular zone extending along the optic stalk.
TS20: cornea	Present		8K/L	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	8M/N	Expression is present in both mitotically active cells, as well as the postmitotic ganglion cells.
TS23: cornea epithelium	Present	Regionally restricted	8M/N	Expression is present in the anterior epithelium of the cornea.
TS23: lens	Present		8M/N

Structure	Level	Pattern	Image	Note
TS23: nasal cavity epithelium	Present		8O/P