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Pdpk1tm1Ejm
Targeted Allele Detail
Summary
Symbol: Pdpk1tm1Ejm
Name: 3-phosphoinositide dependent protein kinase 1; targeted mutation 1, Edward J McManus
MGI ID: MGI:3043781
Synonyms: PDK1(PHki)
Gene: Pdpk1  Location: Chr17:24292654-24369898 bp, - strand  Genetic Position: Chr17, 12.23 cM, cytoband A3.3
Alliance: Pdpk1tm1Ejm page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:90081
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted
Mutation:    Other
 
Mutation details: In order to ablate phosphatidylinositol 3,4,5-trisphoshate, 3 codons in exon 13 encoding arginine residues 472 through 474 were engineered to instead encoded leucince. A single loxP site remained downstream of exon 14 after a floxed neo cassette used for selection was excised via cre-mediated recombination. (J:90081)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 8 assay results
In Structures Affected by this Mutation: 7 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pdpk1 Mutation:  138 strains or lines available
References
Original:  J:90081 McManus EJ, et al., The in vivo role of PtdIns(3,4,5)P(3) binding to PDK1 PH domain defined by knockin mutation. EMBO J. 2004 May 19;23(10):2071-82
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory