Chattm1.1Jrs
Targeted Allele Detail
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| Symbol: |
Chattm1.1Jrs |
| Name: |
choline O-acetyltransferase; targeted mutation 1.1, Joshua R Sanes |
| MGI ID: |
MGI:3045900 |
| Synonyms: |
ChAT- |
| Gene: |
Chat Location: Chr14:32130160-32187866 bp, - strand Genetic Position: Chr14, 19.4 cM
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| Alliance: |
Chattm1.1Jrs page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:91066
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Chattm1Jrs-carring mice were crossed with transgenic mice expressing cre in the germ line to produce this allele in which exons 3 and 4 were replaced with an frt-flanked neo. Southern blot confirmed excision of the exons. RT-PCR revealed the splicing of exon 2 to exon 5, resulting in a frame shift after the codon for amino acid 22 and a stop codon at amino acid 31. Therefore, the protein products were less than 32 amino acids long, instead of the 641, and lacked the catalytic center.
(J:91066)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Chat Mutation: |
58 strains or lines available
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| Original: |
J:91066 Misgeld T, et al., Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase. Neuron. 2002 Nov 14;36(4):635-48 |
| All: |
4 reference(s) |
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Analysis Tools
