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Gene Expression Data
Assay Details
Assay
Reference: J:91447 Gentile L, et al., Single-cell quantitative RT-PCR analysis of Cpt1b and Cpt2 gene expression in mouse antral oocytes and in preimplantation embryos. Cytogenet Genome Res. 2004;105(2-4):215-21
Assay type: RT-PCR
MGI Accession ID: MGI:3047548
Gene symbol: Cpt1b
Gene name: carnitine palmitoyltransferase 1b, muscle
Probe: Cpt1b-pC, Cpt1b-pD
Assay notes: Nested primer sets were used for this analysis. The outer primer set was MGI:3047543. The results illustrated in the paper were obtained using embryos fertilized in vitro, but the authors discuss results obtained with in vivo embryos in the text.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 194 bp Amount Genetic BackgroundMutant Allele(s)Sex
MII P (a) TS28: oocyte Absent Not Specified involves: C3H * C57BL/6 Female
Zyg E0.5 (a) TS1: 1-cell stage conceptus Absent Not Specified involves: C3H * C57BL/6 Not Specified
2-C E1.0 (a) TS2: 2-cell stage conceptus Absent (b) Not Specified involves: C3H * C57BL/6 Not Specified
4-C E2.0 (a) TS3: 4-cell stage conceptus Absent Not Specified involves: C3H * C57BL/6 Not Specified
8-C E2.5 (a) TS3: 8-cell stage conceptus Present (c) Not Specified involves: C3H * C57BL/6 Not Specified
Mor E3.0 (a) TS3: morula-stage conceptus Present Not Specified involves: C3H * C57BL/6 Not Specified
Bl E3.5 (a) TS4: blastocyst-stage conceptus Present Not Specified involves: C3H * C57BL/6 Not Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) In embryos fertilized in vitro expression was found at S-phase.
(c) Expression is present in 22% of embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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