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Gene Expression Data
Assay Details
Assay
Reference: J:86180 Xu M, et al., Enhanced expression of L-type Cav1.3 calcium channels in murine embryonic hearts from Cav1.2-deficient mice. J Biol Chem. 2003 Oct 17;278(42):40837-41
Assay type: RT-PCR
MGI Accession ID: MGI:3050099
Gene symbol: Cacna1d
Gene name: calcium channel, voltage-dependent, L type, alpha 1D subunit
Probe: Dutr01, RACE12
Visualized with: Ethidium bromide
Assay notes: The authors have created more than one mutant allele; it was unclear which was used in this assay.
Results
Image: 3B b ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 649 bp Amount Genetic BackgroundMutant Allele(s)Sex
+/+ E9.5 (a) TS15: heart Present 4 µg; total RNA Not Specified Not Specified
-/- E9.5 (a) TS15: heart Present (b) 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * C57BL/6 Cacna1ctm1Hfm/Cacna1ctm1HfmNot Specified
+/+ E12.5 (a) TS20: heart Present 4 µg; total RNA Not Specified Not Specified
-/- E12.5 (a) TS20: heart Present (c) 4 µg; total RNA involves: 129S1/Sv * 129X1/SvJ * C57BL/6 Cacna1ctm1Hfm/Cacna1ctm1HfmNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression is slightly upregulated in the mutant compared to the wild type.
(c) Expression is upregulated in the mutant compared to the wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/29/2024
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