Tg(TcraTcrb)1100Mjb
Transgene Detail
|
|
| Symbol: |
Tg(TcraTcrb)1100Mjb |
| Name: |
transgene insertion 1100, Michael J Bevan |
| MGI ID: |
MGI:3054907 |
| Synonyms: |
OT-1, OT1, OT1 TCR, OT-I, OTI, OT-I(OVA)TCR, OT-I TCR, OT-ITCR, OVA-tcr-I, OVA-Tg, TCR-OT-I, Tg(OT-I), Tg(TcrOva)1100Mjb, Tg(TcrOVA)1100 mjb |
| Transgene: |
Tg(TcraTcrb)1100Mjb Location: unknown Genetic Position: Chr3, Syntenic
|
| Alliance: |
Tg(TcraTcrb)1100Mjb page
|
|
|
|
| Transgene Type: |
|
Transgenic (Inserted expressed sequence) |
| Mutation: |
|
Insertion
|
| |
|
Tg(TcraTcrb)1100Mjb expresses
2 genes
|
| |
|
Mutation details: Transgenic mice carry two coinjected constructs containing rearranged mouse TCR alpha (Tcra-V2, Tcra-J(alpha26)) and TCR beta (Tcrb-V5, Tcrb-D(beta2), Tcrb-J(beta2.6)) chains isolated from T cell clone 149.42. This transgenic T cell receptor recognizes ovalbumin residues 257-264 (SIINFEKL) in the context of H2Kb. The rearranged Tcra (alpha chain) cDNA specific for ovalbumin was isolated from T cells (clone AD10) and is expressed under the control of a 2-kb fragment of the H-2Kb promoter, a 700-bp fragment including the Ig H chain enhancer located 5' to the promoter, and a 1.7-kb BamHI-PstI fragment containing the 3' half of the human adult 8-globin gene (including part of exon 2, the exon 2-exon 3 C.F6 TCR a-chain gene that was truncated at the 3' XbaI site intron, exon 3) and polyadenylation signal. The Tcrb (beta chain) construct consists of a genomic fragment containing the Tcrb coding and endogenous promoter regions from a T cell clone (B3) specific for ovalbumin.
(J:92867)
|
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 6 strains available
Cell Lines: 0 lines available
|
|
|
|
| Original: |
J:92867 Hogquist KA, et al., T cell receptor antagonist peptides induce positive selection. Cell. 1994 Jan 14;76(1):17-27 |
| All: |
1766 reference(s) |
|
Analysis Tools
