F9tm2(F9)Dws
Targeted Allele Detail
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| Symbol: |
F9tm2(F9)Dws |
| Name: |
coagulation factor IX; targeted mutation 2, Darrel W Stafford |
| MGI ID: |
MGI:3055509 |
| Synonyms: |
R333Q-hFIX |
| Gene: |
F9 Location: ChrX:59044824-59076119 bp, + strand Genetic Position: ChrX, 33.5 cM, cytoband A6-A7
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| Alliance: |
F9tm2(F9)Dws page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:92964
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| Parent Cell Line: |
TC1/TC-1 (ES Cell)
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| Strain of Origin: |
129S6/SvEvTac
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| Allele Type: |
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Targeted |
| Mutation: |
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Insertion
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Mutation details: The targeting construct includes the signal sequence, first intron and propeptide of the mouse gene, the equivalent human cDNA and a neomycin cassette. Two site specific point mutations, T148A and R333Q were created in the human cDNA. The human cDNA is joined to the mouse gene at codons 6 and 7, such that the mouse expresses a missense mutant human protein, but does not express the mouse protein. ELISA analysis using specific human anti-F9 polyclonal antibodies detects human F9 in homozygous mice.
(J:92964)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any F9 Mutation: |
8 strains or lines available
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| Original: |
J:92964 Jin DY, et al., Creation of a mouse expressing defective human factor IX. Blood. 2004 Sep 15;104(6):1733-9 |
| All: |
1 reference(s) |
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Analysis Tools
