Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:83696
Ford-Perriss M, et al., Localisation of specific heparan sulfate proteoglycans during the proliferative phase of brain development. Dev Dyn. 2003 Jun;227(2):170-84
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3522621
|
Gene symbol:
|
Hspg2
|
Gene name:
|
perlecan (heparan sulfate proteoglycan 2)
|
Antibody:
|
906
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
Results
|
Specimen
2A:
embryonic day 10.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forebrain |
Present |
Regionally restricted |
2A
|
Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
|
TS17: hindbrain |
Present |
Regionally restricted |
2A
|
Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
|
TS17: midbrain |
Present |
Regionally restricted |
2A
|
Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
|
Specimen
2A':
embryonic day 10.5
(more )
Specimen
2A':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: telencephalon |
Present |
Regionally restricted |
|
Section through dorsal telencephalon. Two lines of immunoreactivity define the basement membrane lining the neural tube and also the ectoderm.
|
Specimen
2B,2B'':
embryonic day 10.5
(more )
Specimen
2B,2B'':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2C,2C'':
embryonic day 10.5
(more )
Specimen
2C,2C'':
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|