Pax6 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93571 Rowan S, et al., Transdifferentiation of the retina into pigmented cells in ocular retardation mice defines a new function of the homeodomain gene Chx10. Development. 2004 Oct;131(20):5139-52
Assay type:	RNA in situ
MGI Accession ID:	MGI:3525885
Gene symbol:	Pax6
Gene name:	paired box 6

Probe:	Pax6 cDNA14
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen E14.5 heterozygote: embryonic day 14.5; Vsx2^or-J/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		S1M E14.5 orJ/+	Expression is present throughout the inner and outer neuroblastic layers and at high levels in the periphery of the retina (zones 1 and 2).
TS22: pigmented retinal epithelium	Present	Regionally restricted	S1M E14.5 orJ/+	Expression is at high levels in the periphery of the retina (zone 3).

Specimen E14.5 homozygote: embryonic day 14.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		S1M E14.5 orJ/orJ	Expression is present throughout the inner and outer neuroblastic layers and at high levels in the periphery of the retina (zones 1 and 2).
TS22: pigmented retinal epithelium	Present	Regionally restricted	S1M E14.5 orJ/orJ	Expression is present at high levels in the periphery of the retina (zone 3).

Specimen E17.5 heterozygote: embryonic day 17.5; Vsx2^or-J/Vsx2⁺ (more

)

Structure	Level	Pattern	Image	Note
TS25: retina inner nuclear layer	Present	Uniform	S1M E17.5 orJ/+
TS25: retina outer nuclear layer	Present	Uniform	S1M E17.5 orJ/+
TS25: neural retina	Strong	Regionally restricted	S1M E17.5 orJ/+	Expression is present at high levels in the periphery of the retina (zones 1 and 2).
TS25: pigmented retinal epithelium	Strong	Regionally restricted	S1M E17.5 orJ/+	Expression is present at high levels in the periphery of the retina (zone 3).

Specimen E17.5 homozygote: embryonic day 17.5; Vsx2^or-J/Vsx2^or-J (more

)

Structure	Level	Pattern	Image	Note
TS25: retina inner nuclear layer	Present	Uniform	S1M E17.5 orJ/orJ
TS25: retina outer nuclear layer	Present	Uniform	S1M E17.5 orJ/orJ
TS25: neural retina	Strong	Regionally restricted	S1M E17.5 orJ/orJ	Expression is present at high levels in the periphery of the retina (zones 1 and 2).
TS25: pigmented retinal epithelium	Strong	Regionally restricted	S1M E17.5 orJ/orJ	Expression is present at high levels in the periphery of the retina (zone 3).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 06/12/2024 MGI 6.13