Gene Expression Data
Assay Details
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Assay
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Reference:
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J:95790
Richard C, et al., Patency of the preterm fetal ductus arteriosus is regulated by endothelial nitric oxide synthase and is independent of vasa vasorum in the mouse. Am J Physiol Regul Integr Comp Physiol. 2004 Sep;287(3):R652-60
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3528087
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Gene symbol:
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Nos3
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Gene name:
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nitric oxide synthase 3, endothelial cell
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Probe:
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eNOS cDNA
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
d19:
embryonic day 18.5
(more  )
Specimen
d19:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: aorta |
Present |
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2_d19
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Expression was sparse. The compact nature of these vessels prevented distinction between signal localization in the vessel wall and endothelial surface.
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| TS26: ductus arteriosus |
Present |
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2_d19
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Expression was sparse. The compact nature of these vessels prevented distinction between signal localization in the vessel wall and endothelial surface.
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| TS26: pulmonary artery |
Present |
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2_d19
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Expression was sparse. The compact nature of these vessels prevented distinction between signal localization in the vessel wall and endothelial surface.
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Specimen
PP1:
postnatal day 1
(more )
Specimen
PP1:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: ductus arteriosus |
Present |
Regionally restricted |
2_PP1
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Expression was primarily restricted to the endothelial cells in the closed lumen. Additional signals were noted in the wall.
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| TS27: aorta |
Weak |
Regionally restricted |
2_PP1
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Expression is present in the endothelial surface.
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| TS27: pulmonary artery |
Weak |
Regionally restricted |
2_PP1
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Expression is present in the endothelial surface.
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| TS27: cardiac muscle tissue |
Weak |
Uniform |
2_PP1
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Analysis Tools