Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:76363
Lonze BE, et al., Apoptosis, axonal growth defects, and degeneration of peripheral neurons in mice lacking CREB. Neuron. 2002 Apr 25;34(3):371-85
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:3574322
|
|
Gene symbol:
|
Creb1
|
|
Gene name:
|
cAMP responsive element binding protein 1
|
|
Antibody:
|
anti-phosphoCREB
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
The antibody used in this assay only recognizes the protein when it is phosphorylated on Ser-133.
|
|
|
Results
|
Specimen
1B:
embryonic day 11.5; Creb1tm2Gsc/Creb1 +
(more  )
Specimen
1B:
(close  )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Creb1tm2Gsc/Creb1+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: dorsal root ganglion |
Present |
Regionally restricted |
1B
|
Expression is present in the nucleus of the majority of sensory neurons.
|
Specimen
1C:
embryonic day 13.5; Creb1tm2Gsc/Creb1 +
(more )
Specimen
1C:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Creb1tm2Gsc/Creb1+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: dorsal root ganglion |
Present |
Regionally restricted |
1C
|
Expression is present in the nucleus of the majority of sensory neurons.
|
Specimen
1D:
embryonic day 15.5; Creb1tm2Gsc/Creb1 +
(more )
Specimen
1D:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Creb1tm2Gsc/Creb1+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: dorsal root ganglion |
Present |
Regionally restricted |
1D
|
Expression is present in the nucleus of a subset of sensory neurons.
|
Specimen
1E:
(close )
|
Genetic Background:
|
involves: 129P2/OlaHsd * C57BL/6
|
|
Mutant Allele(s):
|
Creb1tm2Gsc/Creb1tm2Gsc
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
1G:
embryonic day 20.5
(more )
Specimen
1G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 20.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: superior cervical ganglion |
Present |
Regionally restricted |
1G
|
Expression is present in the nucleus of the majority of sympathetic neurons.
|
Specimen
1H:
embryonic day 20.5
(more )
Specimen
1H:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 20.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: superior cervical ganglion |
Present |
Regionally restricted |
1H
|
Expression is present in the nucleus of the majority of sympathetic neurons.
|
|
Analysis Tools
