Antibody:	anti-ACLP
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent		1A

Structure	Level	Pattern	Image	Note
TS28: blood vessel	Present	Regionally restricted		Expression is in blood vessels of the uterus (E7.5).

Structure	Level	Pattern	Image	Note
TS28: endometrium	Present		1B	Expression is in reticular fibers. Uterus of E7.5.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		1C

Structure	Level	Pattern	Image	Note
TS15: surface ectoderm	Present		1D
TS15: trunk dermomyotome	Present	Regionally restricted	1D	Expression is on the basal side.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		1E
TS17: yolk sac	Present		1E

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		1F	Shown are smaller vessels in the cephalic mesenchyme.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		1G
TS17: yolk sac	Present		1G
TS17: head mesenchyme	Present	Regionally restricted	1G	Expression is in the condensing mesenchymal cells that form the skull.
TS17: skeleton	Present	Regionally restricted	1G	Expression is in the condensing mesenchymal cells that form the skull.
TS17: surface ectoderm	Present	Regionally restricted	1G	Expression is in the skin connective tissue.
TS17: nervous system	Absent		1G

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present		1H
TS17: yolk sac	Present		1H	Expression is throughout the membranes and is strong in the vasculature.

Structure	Level	Pattern	Image	Note
TS19: embryo	Weak	Uniform	1I

Structure	Level	Pattern	Image	Note
TS19: internal carotid artery	Present		1J
TS19: notochord	Present		1J

Structure	Level	Pattern	Image	Note
TS19: internal carotid artery	Present		1K

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present		1L

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted	2A	Expression is in numerous sites undergoing organogenesis.

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Regionally restricted	2B	Expression is in numerous sites undergoing organogenesis.

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Strong	Regionally restricted	2C	Expression is in the smooth muscle layer.

Structure	Level	Pattern	Image	Note
TS21: lung	Strong	Regionally restricted	2D	Expression is in the airway basement membrane.

Structure	Level	Pattern	Image	Note
TS21: blood vessel	Strong	Regionally restricted	2E	Expression is in the smaller blood vessels supplying the skin.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present	Regionally restricted	2F	Expression is in the perichondrium of the vertebrae.
TS21: dorsal root ganglion	Absent		2F

Structure	Level	Pattern	Image	Note
TS21: umbilical artery	Present	Regionally restricted	2G	Expression is in the smooth muscle layer.
TS21: trunk mesenchyme	Present	Regionally restricted	2G	Expression is in the connective tissue in the umbilical ring.

Structure	Level	Pattern	Image	Note
TS21: medullary tubule	Present		2H
TS21: adrenal gland	Present		2H	Expression is in the adrenal primordium.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present		3A

Structure	Level	Pattern	Image	Note
TS24: vault of skull	Present	Regionally restricted	3B	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: dermis	Very strong		3C
TS24: epidermis	Absent		3C

Structure	Level	Pattern	Image	Note
TS24: esophagus	Present	Regionally restricted	3E	Expression is in the submucosal layer of the esophagus, absent in the muscular layer.
TS24: esophagus epithelium	Absent		3E

Structure	Level	Pattern	Image	Note
TS24: blood vessel	Present		3G	Expression in smaller vessels supplying the musculature.
TS24: embryo mesenchyme	Present	Regionally restricted	3G	Expression is in the tendon.

Structure	Level	Pattern	Image	Note
TS24: lung	Present		3H	Expression is in the airway basement membrane.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present		3I
TS24: falciform ligament	Present		3I
TS24: epidermis	Absent		3I

Structure	Level	Pattern	Image	Note
TS24: rib	Very strong	Regionally restricted	3J	Expression is in the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Regionally restricted	3K	Expression is in the submucosa.

Structure	Level	Pattern	Image	Note
TS24: tongue	Present	Regionally restricted	3L	Expression is in the endomysial fibers.

Structure	Level	Pattern	Image	Note
TS24: choroid plexus	Present		3M

Structure	Level	Pattern	Image	Note
TS24: tail mesenchyme	Very strong	Regionally restricted	3N	Expression is in tendons and diffusely throughout the connective tissue.