Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Tumor data | Find Mice (IMSR) | References

Symbol:	Atg7tm1Tchi
Name:	autophagy related 7; targeted mutation 1, Tomoki Chiba
MGI ID:	MGI:3587769
Synonyms:	Atg7F, Atg7Flox, ATG7flox
Gene:	Atg7  Location: Chr6:114620075-114837565 bp, + strand  Genetic Position: Chr6, 53.05 cM, cytoband E3
Alliance:	Atg7tm1Tchi page

Germline Transmission:	Earliest citation of germline transmission: J:100199
Parent Cell Line:	TT2 (ES Cell)
Strain of Origin:	(C57BL/6NCrlj x CBA/JNCrlj)F1

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A targeting construct was designed to insert a loxP site in intron of the 13th coding exon. Coding exon 14 was fused to a cDNA encoding coding exons 15, 16, and 17 together with a polyadenylation signal after the stop codon. This was then followed by a neomycin resistance cassette and a second loxP site. An additional exon 14 sequence with a stop codon preceding the active site cysteine codon was inserted after the second loxP site. Immunoblot showed that the presence of the loxP sites and neo did not interefere with translation. (J:100199)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	17 anatomical structure(s)

List all tumor models in MMHCdb carrying Atg7tm1Tchi

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Atg7 Mutation:	51 strains or lines available

Original:	J:100199 Komatsu M, et al., Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 2005 May 9;169(3):425-34
All:	350 reference(s)