Gene Expression Data
Assay Details
Assay
|
Reference:
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J:99372
van der Zwaag B, et al., Identifying new candidate genes for hereditary facial paresis on chromosome 3q21-q22 by RNA in situ hybridization in mouse. Genomics. 2005 Jul;86(1):55-67
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3605538
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Gene symbol:
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Podxl2
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Gene name:
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podocalyxin-like 2
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Probe:
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Podxl2 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
|
Alkaline phosphatase
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Assay notes:
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After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde. The author divided the genes analyzed in this publication into 4 groups based on their expression patterns. The expression pattern of this gene was placed in group 4 (See Table 1).
|
|
Results
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Specimen
6A, 6B:
embryonic day 12.5
(more )
Specimen
6A, 6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cerebellum primordium |
Present |
Regionally restricted |
6A
,
6B
|
Expression was detected in the dorsal cells of the cerebellar primordium.
|
TS20: spinal cord |
Present |
|
6A
,
6B
|
|
TS20: medulla oblongata |
Present |
Regionally restricted |
6B
,
6A
|
Expression was detected in the anterior part of the medulla oblongata but was absent from the neuroepithelium.
|
TS20: hindbrain |
Present |
Regionally restricted |
6A
,
6B
|
Expression was detected in the neuroepithelium.
|
TS20: midbrain |
Present |
Regionally restricted |
6B
,
6A
|
Expression was detected in the neuroepithelium.
|
TS20: central nervous system |
Present |
Regionally restricted |
6A
,
6B
|
Expression was detected in the neuroepithelium of the midbrain and hindbrain, other regions of the central nervous system were void of expression in the neuroepithelium.
|
Specimen
6C:
embryonic day 14.5
(more )
Specimen
6C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
6D:
embryonic day 14.5
(more )
Specimen
6D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: gut |
Present |
Regionally restricted |
6D
|
Expression was detected in the gut wall smooth muscle cells.
|
Specimen
6E:
embryonic day 14.5
(more )
Specimen
6E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
6F:
embryonic day 16.5
(more )
Specimen
6F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
6G:
embryonic day 18.5
(more )
Specimen
6G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Fresh Frozen
|
Embedding:
|
Cryosection
|
|