Gene Expression Data
Assay Details
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Assay
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Reference:
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J:11916
Oh SP, et al., Tissue-specific expression of type XII collagen during mouse embryonic development. Dev Dyn. 1993 Jan;196(1):37-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3612413
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Gene symbol:
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Col12a1
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Gene name:
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collagen, type XII, alpha 1
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Antibody:
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anti-a1(XII) collagen
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Younger embryos (E8.5-15.5) were fixed in 100% ethanol containing 4% polyvinylpyrolidone; older embryos and newborns were fixed in ethanol-PVP supplemented with 20% dimethyl sulfoxide to allow better infiltration of tissues.
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Results
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Specimen
Not shown:
embryonic day 8.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
Not shown:
embryonic day 9.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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CD-1
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Age:
|
embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
Not shown:
embryonic day 10.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
2A:
embryonic day 12.5
(more )
Note: This is a sagittal section.
Specimen
2A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This is a sagittal section.
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Specimen
2B:
embryonic day 16.5
(more )
Note: This is a sagittal section. Fig. 2C and the inset in Fig. 2B are higher magnification images of Fig. 2B.
Specimen
2B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This is a sagittal section. Fig. 2C and the inset in Fig. 2B are higher magnification images of Fig. 2B.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: intervertebral disc |
Strong |
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2B
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There is intense staining of the fibrocartilaginous intervertebral discs; the staining is in the pericellular extracellular matrix, not the cell body. Inset shows staining at higher magnification.
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| TS24: trunk mesenchyme |
Present |
Regionally restricted |
2B
,
2C
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Staining is present in the perichondrium of the vertebral bodies, as well as in associated ligaments.
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| TS24: vertebra |
Absent |
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2C
,
2B
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There is no staining in structures composed of hyaline cartilage.
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| TS24: nucleus pulposus |
Absent |
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2C
,
2B
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| TS24: dermis |
Present |
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2B
,
2C
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Specimen
2D left:
embryonic day 16.5
(more )
Note: This is a transverse section.
Specimen
2D left:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This is a transverse section.
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Specimen
2D right:
embryonic day 16.5
(more )
Note: This is a transverse section.
Specimen
2D right:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This is a transverse section.
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Specimen
2E:
embryonic day 17.5
(more )
Specimen
2E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
2F:
postnatal day 1
(more )
Specimen
2F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3A:
embryonic day 16.5
(more )
Note: This is a sagittal section.
Specimen
3A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Note:
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This is a sagittal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cranium |
Present |
Regionally restricted |
3B
,
3A
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Expression is present in membranous bone; there is intense staining in the perichondrium.
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| TS24: clavicle |
Present |
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3B
,
3A
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| TS24: Meckel's cartilage |
Absent |
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3B
,
3A
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There is no expression in structures composed of hyaline cartilage, such as Meckel's cartilage and the hyoid cartilage.
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| TS24: cartilage |
Absent |
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3B
,
3A
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There is no expression in structures composed of hyaline cartilage, such as Meckel's cartilage and the hyoid cartilage.
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| TS24: hyoid |
Absent |
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3B
,
3A
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There is no expression in structures composed of hyaline cartilage, such as Meckel's cartilage and the hyoid cartilage.
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Specimen
3C:
embryonic day 16.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: blood vessel |
Present |
Regionally restricted |
3C
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There is expression in the tunica media and adventitia layers; the intima and endothelial layers are unstained.
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Specimen
3D:
embryonic day 11.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
Not shown:
embryonic day 11.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: blood vessel |
Present |
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| TS19: limb |
Present |
Regionally restricted |
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Expression is present under the apical endodermal ridge of the limb paddles.
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Specimen
3E:
embryonic day 16.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: dermis |
Present |
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3E
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Expression is present in the dermis, the connective tissue of the hair follicles, and subcutaneous dense connective tissue.
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| TS24: epidermis |
Absent |
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3E
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Specimen
3F:
postnatal day 1
(more )
Specimen
3F:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: dermis |
Present |
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3F
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Expression is present in the dermis and subcutaneous dense connective tissue.
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| TS27: hair follicle |
Present |
Regionally restricted |
3F
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Expression is present in the connective tissue of the hair follicles.
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| TS27: epidermis |
Absent |
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3F
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Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
4B:
embryonic day 16.5
(more )
Specimen
4B:
(close )
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Genetic Background:
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CD-1
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Age:
|
embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
Not shown:
embryonic day 16.5
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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CD-1
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Age:
|
embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
4C:
postnatal adult
(more )
Specimen
4C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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