Probe:	Alk cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present
TS17: facial ganglion	Present
TS17: 4th ventricle	Present	Regionally restricted		Expression is in the ventral part.

Structure	Level	Pattern	Image	Note
TS19: 2nd branchial arch	Present
TS19: diencephalon	Present
TS19: esophagus	Present
TS19: facial ganglion	Present
TS19: inferior vagus ganglion	Present
TS19: stomach	Present
TS19: 4th ventricle	Present	Regionally restricted		Expression is in the ventral part.
TS19: future spinal cord	Present	Regionally restricted		Expression is ventral.
TS19: olfactory epithelium	Present	Regionally restricted		Expression is in the ventral part of the olfactory epithelium.
TS19: head mesenchyme	Present	Regionally restricted		Expression is in the area between the nasal pit and lateral ventricle.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex subventricular zone	Present
TS20: cerebral cortex ventricular layer	Present
TS20: diencephalon	Present
TS20: dorsal aorta	Present
TS20: endolymphatic sac	Present
TS20: esophagus	Present
TS20: facial ganglion	Present
TS20: inferior vagus ganglion	Present
TS20: medulla oblongata	Present
TS20: midbrain roof plate	Present
TS20: midgut	Present
TS20: pons	Present
TS20: posterior semicircular canal	Present
TS20: spinal cord	Present
TS20: stomach	Present
TS20: sympathetic nervous system	Present
TS20: tongue	Present
TS20: vestibulo-cochlear ganglion	Present
TS20: vibrissa	Present
TS20: vomeronasal organ	Present
TS20: 4th ventricle	Present	Regionally restricted		Expression is in the ventral part.
TS20: midbrain	Present	Regionally restricted		Expression is in the tegmentum.
TS20: utricle	Present	Regionally restricted		Expression is in the lateral semicircular canal.
TS20: optic chiasma	Present	Regionally restricted		Expression is in the dorsal epithelium in the region of the optic chiasm.
TS20: olfactory epithelium	Present	Regionally restricted		Expression is in the ventral part of the olfactory epithelium.
TS20: head mesenchyme	Present	Regionally restricted		Expression is in the area between the nasal pit and lateral ventricle and outside the most posterior part of the nasal cavity.
TS20: embryo mesenchyme	Present	Regionally restricted		Expression is in the premuscle mesodermal condensations.
TS20: extraembryonic component	Present	Regionally restricted		Expression is in the umbilical cord.
TS20: forelimb	Present	Regionally restricted		Elongated stain in the limbs.
TS20: hindlimb	Present	Regionally restricted		Elongated stain in the limbs.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Present
TS21: cerebral cortex ventricular layer	Present
TS21: diencephalon	Present
TS21: dorsal aorta	Present
TS21: endolymphatic sac	Present
TS21: esophagus	Present
TS21: facial ganglion	Present
TS21: inferior vagus ganglion	Present
TS21: lateral semicircular canal	Present
TS21: medulla oblongata	Present
TS21: midbrain roof plate	Present
TS21: midgut	Present
TS21: pons	Present
TS21: posterior semicircular canal	Present
TS21: spinal cord	Present
TS21: stomach	Present
TS21: sympathetic ganglion	Present
TS21: tongue	Present
TS21: vestibulo-cochlear ganglion	Present
TS21: vomeronasal organ	Present
TS21: 4th ventricle	Present	Regionally restricted		Expression is in the ventral part.
TS21: midbrain	Present	Regionally restricted		Expression is in the tegmentum.
TS21: trigeminal nerve	Present	Regionally restricted		Expression is in the maxillary division of the trigeminal (V) nerve.
TS21: autonomic nervous system	Present	Regionally restricted		Expression is in the ciliary ganglion.
TS21: olfactory epithelium	Present	Regionally restricted		Expression is in the ventral part of the olfactory epithelium.
TS21: head mesenchyme	Present	Regionally restricted		Expression is outside the most posterior part of the nasal cavity.
TS21: skeletal muscle	Present	Regionally restricted		Expression is in the long muscle of the neck.
TS21: forelimb	Present	Regionally restricted		Elongated stain in the limbs.
TS21: hindlimb	Present	Regionally restricted		Elongated stain in the limbs.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex subventricular zone	Present
TS22: cerebral cortex ventricular layer	Present
TS22: diencephalon	Present
TS22: dorsal aorta	Present
TS22: esophagus	Present
TS22: inferior vagus ganglion	Present
TS22: medulla oblongata	Present
TS22: midbrain roof plate	Present
TS22: midbrain tegmentum	Present
TS22: midgut	Present
TS22: neural retinal epithelium	Present
TS22: pons	Present
TS22: spinal cord	Present
TS22: stomach	Present
TS22: sympathetic ganglion	Present
TS22: tongue	Present
TS22: vestibulo-cochlear ganglion	Present
TS22: vomeronasal organ	Present
TS22: trigeminal nerve	Present	Regionally restricted		Expression is in the maxillary division of the trigeminal (V) nerve.
TS22: olfactory epithelium	Present	Regionally restricted		Expression is in the ventral part of the olfactory epithelium.
TS22: skeletal muscle	Present	Regionally restricted		Expression is in the long muscle of the neck.
TS22: forelimb	Present	Regionally restricted		Elongated stain in the limbs.
TS22: hindlimb	Present	Regionally restricted		Elongated stain in the limbs.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex subventricular zone	Present
TS24: diencephalon	Present
TS24: dorsal root ganglion	Present
TS24: sublingual gland	Present
TS24: submandibular gland	Present
TS24: vestibulo-cochlear ganglion	Present
TS24: trigeminal nerve	Present	Regionally restricted		Expression is in the rootlets and the ophthalmic division of the trigeminal (V) nerve.
TS24: sympathetic ganglion	Present	Regionally restricted		Expression is in the lumbar sympathetic ganglion.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall	Present	Regionally restricted	1a	Expression is in the wall of diencephalon at the level of infundibular recess.

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present	Regionally restricted	1b	Expression is in a specific cell population.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1c	Expression is in the dorsal part of the lateral ventricle wall, this area presumably corresponds to part of the amygdala.

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present		1d
TS22: telencephalon	Present	Regionally restricted	1d	Expression is in the subventricular zone and is absent in the intermediate zone.
TS22: cortical plate	Absent		1d
TS22: telencephalon marginal layer	Absent		1d

Structure	Level	Pattern	Image	Note
TS17: hindbrain lateral wall	Present	Regionally restricted	1e	Expression is in the ventral part.

Structure	Level	Pattern	Image	Note
TS20: midbrain roof plate	Present		1f
TS20: midbrain	Present		1f

Structure	Level	Pattern	Image	Note
TS20: posterior semicircular canal	Present		2a
TS20: utricle	Present	Regionally restricted	2a	Expression is in the lateral semicircular canal.

Structure	Level	Pattern	Image	Note
TS21: endolymphatic sac	Present		2b
TS21: vestibulo-cochlear ganglion	Present		2b

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Present		2c

Structure	Level	Pattern	Image	Note
TS20: duodenum caudal part mesenchyme	Strong	Regionally restricted	2d	Expression is surrounding the esophagus, stomach and midgut (duodenum).
TS20: esophagus mesenchyme	Strong	Regionally restricted	2d	Expression is surrounding the esophagus, stomach and midgut (duodenum).
TS20: stomach mesenchyme	Strong	Regionally restricted	2d	Expression is surrounding the esophagus, stomach and midgut (duodenum).

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present		2e

Structure	Level	Pattern	Image	Note
TS21: autonomic nervous system	Present	Regionally restricted	2f	Expression is in the ciliary ganglion.

Structure	Level	Pattern	Image	Note
TS19: neural tube marginal layer	Present		3a

Structure	Level	Pattern	Image	Note
TS20: sympathetic nervous system	Present		3b

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present	Regionally restricted	3c	Expression in the spinal cord switches from a ventral stain rostrally to a dorsal stain caudally.
TS21: tail spinal cord	Present	Regionally restricted	3c	Expression in the spinal cord switches from a ventral stain rostrally to a dorsal stain caudally.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		3d
TS20: vomeronasal organ	Present		3d

Structure	Level	Pattern	Image	Note
TS20: head mesenchyme	Present	Regionally restricted	3e	Expression is ventral to the nasal cavity and posterior to the lateral ventricle of the brain.

Structure	Level	Pattern	Image	Note
TS21: nose	Present	Regionally restricted	3f	Expression is dorsal to the nasal epithelium.

Structure	Level	Pattern	Image	Note
TS22: tongue	Present	Regionally restricted	3g	Expression is below the intrinsic muscle component of the tongue.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted		Expression is in the neuroepithelium surrounding the infundibular recess of diencephalon.
TS17: midbrain	Absent

Structure	Level	Pattern	Image	Note
TS19: 4th ventricle	Present	Regionally restricted		Expression is in the ventral part.
TS19: midbrain	Absent

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present
TS21: midbrain roof plate	Present

Structure	Level	Pattern	Image	Note
TS22: midbrain	Present
TS22: midbrain roof plate	Present

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Absent
TS23: cerebral cortex ventricular layer	Absent

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Absent
TS24: midbrain	Absent

Structure	Level	Pattern	Image	Note
TS24: diencephalon	Weak		S1a

Structure	Level	Pattern	Image	Note
TS20: optic chiasma	Present	Regionally restricted	S1b	Expression is in the dorsal epithelium.

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present		S1c

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		S1d
TS21: vestibulo-cochlear ganglion	Present		S1d

Structure	Level	Pattern	Image	Note
TS22: midgut loop	Present		S1e

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		S1f

Structure	Level	Pattern	Image	Note
TS21: skeletal muscle	Present	Regionally restricted	S1g	Expression is in the area covering the long muscles of neck.

Structure	Level	Pattern	Image	Note
TS22: hindlimb	Present	Regionally restricted	S1h	Expression is like a protrusion in the limb.

Structure	Level	Pattern	Image	Note
TS22: dorsal aorta	Strong		S1i