Apob<tm1Mae> Targeted Allele Detail MGI Mouse (MGI:3665449)

Apob^tm1Mae
Targeted Allele Detail

Summary

Symbol:	Apob^tm1Mae
Name:	apolipoprotein B; targeted mutation 1, Nobuyo Maeda
MGI ID:	MGI:3665449
Synonyms:	apo b81
Gene:	Apob Location: Chr12:8027648-8066835 bp, + strand Genetic Position: Chr12, 3.53 cM
Alliance:	Apob^tm1Mae page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:36426
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Mutation
description

Allele Type:		Targeted
Mutation:		Insertion
		Mutation details: Using an 'in step' recombination strategy, the sequence encoding the putative binding domains in exon 26 were replaced with those encoding human HBB peptides. The 5' binding domain (3143LSVKAQYKKNSD3154) was replaced with amino acids 1-12 of the HBBS globin (1VHLTPVEKSAVT12). The 3' binding domain (3356GTSRLMRKRGL3366) was replaced with amino acids 120-131 of HBBA globin (120KEFTPPVQAAYQ131). The reading frame was maintained in the mutant allele, however, the disruption creates a premature stop codon at position 3688. The mutant allele contains 3687 residues, which is 81% of the full length protein. (J:36426)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Disease models

Expression

In Structures Affected by this Mutation:

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available Cell Lines: 0 lines available
Carrying any Apob Mutation:	224 strains or lines available

References

Original:	J:36426 Toth LR, et al., Two distinct apolipoprotein B alleles in mice generated by a single 'in-out' targeting. Gene. 1996 31;178(1-2):161-8
All:	3 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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