Probe:	Disc1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Sections were post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS24: brain	Strong		1_Antisense E16

Structure	Level	Pattern	Image	Note
TS27: brain	Strong		1_Antisense P1

Structure	Level	Pattern	Image	Note
TS28: brain	Strong		1_Antisense P21

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	2_Rostral Hippocampus E14	Expression was strong in the rostral region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	2_Central Hippocampus E14	Expression was strong in the central region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	2_Caudal Hippocampus E14	Expression was strong in the caudal region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	2_Rostral Hippocampus E16	Expression was strong in the rostral region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	2_Central Hippocampus E16	Expression was strong in the central region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	2_Caudal Hippocampus E16	Expression was strong in the caudal region of the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2_Rostral Hippocampus E18	Expression was strong in the rostral region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2_Central Hippocampus E18	Expression was strong in the central region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	2_Caudal Hippocampus E18	Expression was strong in the caudal region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	2_Rostral Hippocampus P1	Expression was strong in the rostral region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	2_Central Hippocampus P1	Expression was strong in the central region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	2_Caudal Hippocampus P1	Expression was strong in the caudal region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	2_Rostral Hippocampus P21	Expression was strong in the rostral region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	2_Central Hippocampus P21	Expression was strong in the central region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	2_Caudal Hippocampus P21	Expression was strong in the caudal region of the hippocampus.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb glomerular layer	Present	Patchy	3A	Expression was detected in scattered cells.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Patchy	3B	Expression was detected in the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Patchy	3C	Expression was detected in the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		3D
TS27: thalamus	Present		3D
TS27: cerebral cortex	Present	Patchy	3D	Expression was detected in the cortical plate.
TS27: hippocampus CA1	Strong		3D
TS27: hippocampus CA2	Strong		3D
TS27: hippocampus CA3	Strong		3D
TS27: dentate gyrus	Weak		3D

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		3E
TS27: thalamus	Present		3E
TS27: cerebral cortex	Present	Patchy	3E	Expression was detected in the cortical plate.
TS27: hippocampus CA1	Strong		3E
TS27: hippocampus CA2	Strong		3E
TS27: hippocampus CA3	Strong		3E
TS27: dentate gyrus	Weak		3E

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Present		3F
TS27: cerebral cortex	Present	Patchy	3F	Expression was detected in the cortical plate.
TS27: hippocampus CA1	Strong		3F
TS27: hippocampus CA2	Strong		3F
TS27: hippocampus CA3	Strong		3F
TS27: dentate gyrus	Weak		3F

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Present		3G

Structure	Level	Pattern	Image	Note
TS28: main olfactory bulb glomerular layer	Present	Patchy	4A	Expression was detected in scattered cells.

Structure	Level	Pattern	Image	Note
TS28: brain	Present		4B

Structure	Level	Pattern	Image	Note
TS28: brain	Present		4C

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		4D
TS28: cerebral cortex	Present	Patchy	4D	Expression was detected in scattered cells in the superficial laminae of the cerebral cortex.
TS28: hippocampus CA1	Present	Patchy	4D	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA2	Present	Patchy	4D	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA3	Present	Patchy	4D	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		4E
TS28: cerebral cortex	Present	Patchy	4E	Expression was detected in scattered cells in the superficial laminae of the cerebral cortex.
TS28: hippocampus CA1	Present	Patchy	4E	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA2	Present	Patchy	4E	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA3	Present	Patchy	4E	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		4F
TS28: hippocampus CA1	Present	Patchy	4F	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA2	Present	Patchy	4F	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.
TS28: hippocampus CA3	Present	Patchy	4F	Expression was detected in scattered cells of the CA1-3 pyramidal neurons of Ammon's horn.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus granule cell layer	Strong		4G

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Patchy	4H	Expression was detected in scattered cells of the granule cell layer of the cerebellum.

Structure	Level	Pattern	Image	Note
TS22: telencephalon	Strong	Regionally restricted	5A	Expression was detected in the rostral hippocampal formation, predominantly in cells that are migrating or have reached their mature positions rather than those in the proliferative zones.

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	5B	Expression was detected in the rostral hippocampal formation, predominantly in cells that are migrating or have reached their mature positions rather than those in the proliferative zones.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	5C	Expression was detected in the rostral hippocampal formation. Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS26: dentate gyrus	Weak		5C

Structure	Level	Pattern	Image	Note
TS27: hippocampus	Strong	Regionally restricted	5D	Expression was detected in the rostral hippocampal formation. Both the level of expression and pattern of label change with time in the dentate gyrus and pyramidal neurons of Ammon's horn.
TS27: hippocampus CA1	Strong		5D	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS27: hippocampus CA2	Strong		5D	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS27: hippocampus CA3	Strong		5D	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS27: dentate gyrus	Weak		5D

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Strong	Regionally restricted	5E	Expression was detected in the rostral hippocampal formation. Both the level of expression and pattern of label change with time in the dentate gyrus and pyramidal neurons of Ammon's horn.
TS28: hippocampus CA1	Weak		5E	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS28: hippocampus CA2	Weak		5E	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS28: hippocampus CA3	Weak		5E	Expression in the CA1-3 pyramidal cells of Ammon's horn was highest around birth and attenuated with time.
TS28: dentate gyrus	Strong		5E

Structure	Level	Pattern	Image	Note
TS24: cortical subplate	Present		6A

Structure	Level	Pattern	Image	Note
TS24: telencephalon	Strong	Regionally restricted	6B	Expression was strong in the hippocampus, predominantly in cells that are migrating or have reached their mature positions rather than those in the proliferative zones.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Strong	Regionally restricted	6C inset , 6C	Expression was strong in the hippocampus, predominantly in cells that are migrating or have reached their mature positions rather than those in the proliferative zones.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	6D	Expression was scattered throughout the bed nucleus of the stria terminalis.

Structure	Level	Pattern	Image	Note
TS26: diencephalon	Present	Regionally restricted	6E	Expression was scattered throughout the bed nucleus of the stria terminalis.

Structure	Level	Pattern	Image	Note
TS26: thalamus	Present	Regionally restricted	6F , 6F inset	Expression was scattered throughout the reuniens thalamic nucleus.

Structure	Level	Pattern	Image	Note
TS27: cerebellum granule cell layer	Present		6G

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		6H

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		6I
TS28: cerebellum Purkinje cell layer	Absent		6I

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	7A	Expression was detected in the dorsal lateral developing cortex. The level of expression and pattern of label in the laminae changes between E14 and P21.
TS22: cerebral cortex marginal layer	Strong		7A
TS22: cortical subplate	Strong		7A

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	7B	Expression was detected in the dorsal lateral developing cortex. The level of expression and pattern of label in the laminae changes between E14 and P21.
TS24: cerebral cortex marginal layer	Present		7B
TS24: cortical subplate	Present		7B
TS24: cerebral cortex subventricular zone	Strong		7B

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	7C	Expression was detected in the dorsal lateral developing cortex. The level of expression and pattern of label in the laminae changes between E14 and P21.
TS26: cortical subplate	Present		7C
TS26: cortical plate	Present	Regionally restricted	7C	Expression was restricted to the deep laminae of the cortical plate.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	7D	Expression was seen throughout the thickness of the cortex. The level of expression and pattern of label in the laminae changes between E14 and P21.
TS27: cortical layer II	Strong		7D
TS27: cortical layer III	Strong		7D

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	7E	The level of expression and pattern of label in the laminae changes between E14 and P21.