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Chattm1(cre)Lowl
Targeted Allele Detail
Summary
Symbol: Chattm1(cre)Lowl
Name: choline O-acetyltransferase; targeted mutation 1, Bradford B Lowell
MGI ID: MGI:3689420
Synonyms: ChAT-IRES-Cre
Gene: Chat  Location: Chr14:32130160-32187866 bp, - strand  Genetic Position: Chr14, 19.4 cM
Alliance: Chattm1(cre)Lowl page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:114556
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Chattm1(cre)Lowl expression driven by 1 gene
 
Mutation details: An optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. The neo cassette was excised with Flp recombinase. Cre recombinase activity is reported in all cholinergic neurons. This allele generated from Chattm2(cre)Lowl. (J:114556, J:169562)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chat (mouse)
Summary of all recombinase alleles driven by Chat.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  58 strains or lines available
References
Original:  J:114556 Lowell BB, et al., Development and phenotype of ChAT-IRES-Cre mice. MGI Direct Data Submission. 2006;
All:  142 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory