Rest RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:100382 Qiang M, et al., Neuron-restrictive silencer factor regulates the N-methyl-D-aspartate receptor 2B subunit gene in basal and ethanol-induced gene expression in fetal cortical neurons. Mol Pharmacol. 2005 Jun;67(6):2115-25
Assay type:	RT-PCR
MGI Accession ID:	MGI:3699010
Gene symbol:	Rest
Gene name:	RE1-silencing transcription factor

Probe:	NRSF-S, NRSF-AS-2
Probe preparation:	labelled with SYBR green
Assay notes:	This assay was a quantitative real-time RT-PCR. The results were presented in a graph.

Results

Image: 7B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	201 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E14	E14.0 (a)	TS22: cerebral cortex	Present	1 µg; total RNA	C57BL/6		Not Specified
P0	P0	TS27: cerebral cortex	Present	1 µg; total RNA	C57BL/6		Not Specified
P7	P7	TS28: cerebral cortex	Present	1 µg; total RNA	C57BL/6		Not Specified
P14	P14	TS28: cerebral cortex	Present	1 µg; total RNA	C57BL/6		Not Specified
P30	P30	TS28: cerebral cortex	Weak	1 µg; total RNA	C57BL/6		Not Specified
P90	P90	TS28: cerebral cortex	Weak	1 µg; total RNA	C57BL/6		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was highest at E14, decreased rapidly until P7, increased slightly at P14, then fell again gradually. Expression level in the adult was 10% that of the E14 sample.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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