Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
|
Assay type:
|
In situ reporter (knock in)
|
MGI Accession ID:
|
MGI:3699628
|
Gene symbol:
|
Hoxc8
|
Gene name:
|
homeobox C8
|
Reporter:
|
lacZ
|
|
Direct Detection
|
|
Results
|
Specimen
2A:
embryonic day 9.0; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
2A:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2B:
embryonic day 9.0; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
2B:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: neural ectoderm |
Present |
Regionally restricted |
2B
|
From the rostral region of the embryo to the posterior neuropore, expression was detected in the neurogenic ependymal layer.
|
Specimen
2C:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
2C:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
2C
|
The rostral limit of expression was at the level of the 8th pair of somites.
|
TS20: trunk mesenchyme |
Present |
Regionally restricted |
2C
|
Expression was detected in paraxial mesoderm posteriorly to the first thoracic vertebra. The rostral limit of expression was at the level of the 13th pair of somites.
|
TS20: forelimb |
Present |
Regionally restricted |
2C
|
Expression was detected in the rostral-half of the proximal forelimb bud.
|
Specimen
2D:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
2D:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Graded |
2D
|
Expression was detected in a gradient in the ventral horn from C6 to C8-T1, in which the labeling reaches its highest level. Between 3rd to 5th cervical vertebrae, a small number of cells expressed b-gal at a low level in the intermediate zone.
|
Specimen
2F:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay MGI:2149091).
Specimen
2F:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay MGI:2149091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: basal columns |
Present |
|
2F
|
Expression was detected in the motor columns. The specimen was from the level of the eighth cervical vertebra (C8).
|
Specimen
2G:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
2G:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
2H:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: basal columns |
Present |
|
2H
|
Expression was detected in cells, as expected, and their gross pattern of distribution along the mediolateral axis was not significantly modified.
|
Specimen
3I - C6:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3I - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3I
|
The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.
|
Specimen
3J - C7:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3J - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3J
|
The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons. In the medial and caudal parts of this level, expression was in 100% of the motor neurons.
|
Specimen
3K - C8 LMC:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3K - C8 LMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3K
|
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons in the lateral motor column.
|
Specimen
3L - C8 MMC:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3L - C8 MMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3L
|
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in 100% of somatic motor neurons in the medial motor column.
|
Specimen
3M - C6:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3M - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3M
|
The specimen was from the level of the cervical vertebra 6. At this level, expression was restricted to interneurons and/or glial cells.
|
Specimen
3N - C7:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3N - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3N
|
The specimen was from the level of the cervical vertebra 7. At the rostral part of this level, expression was detected in a mosaic population of motor neurons.
|
Specimen
3O - C8 LMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3O - C8 LMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3O
|
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the lateral motor column of the heterozygous mutant.
|
Specimen
3P - C8 MMC:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
Specimen
3P - C8 MMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: turquoise/blue - Hoxc8; brown - Isl1 (assay MGI:2149089) and Isl2 (assay Acc(MGI:2149091||)).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3P
|
The specimen was from the level of the cervical vertebra 8. At this level, expression was detected in somatic motor neurons (but not all) in the medial motor column of the heterozygous mutant.
|
|