Gene Expression Data
Assay Details
Assay
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Reference:
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J:45780
Tiret L, et al., Increased apoptosis of motoneurons and altered somatotopic maps in the brachial spinal cord of Hoxc-8-deficient mice. Development. 1998 Jan;125(2):279-91
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3700202
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-C-Isl-1 protein (K5)
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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Antibody used detects both Isl1 and Isl2; both genes have been annotated.
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Results
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Specimen
3A - C6:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3A - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3A
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
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Specimen
3B - C7:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3B - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3B
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
|
Specimen
3C - C8:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3C - C8:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
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Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3C
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
3D - T1:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3D - T1:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
3D
|
Expression was detected in motor neurons. The specimen was from the level of the thoracic vertebra 1.
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Specimen
3E - C6:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3E - C6:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
3E
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 6.
|
Specimen
3F - C7:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3F - C7:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
3F
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 7.
|
Specimen
3G - C8 LMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3G - C8 LMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
3G
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Expression was detected in motor neurons in the lateral motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
3H - C8 MMC:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
Specimen
3H - C8 MMC:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
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Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - Hoxc8 (assay MGI:2149088).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
3H
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Expression was detected in motor neurons in the medial motor column. The specimen was from the level of the cervical vertebra 8.
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Specimen
4C:
embryonic day 12.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4C:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Single cells |
4C
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4D:
embryonic day 12.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4D:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Single cells |
4D
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4G:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4G:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
4G
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4H:
embryonic day 13.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4H:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
4H
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4I:
embryonic day 13.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Specimen
4I:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
4I
|
Expression was detected in motor neurons.
|
Specimen
4J:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 13.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Present |
Single cells |
4J
|
Expression was detected in motor neurons, but there were fewer motor neurons in the mutant.
|
Specimen
4K:
embryonic day 14.5; Hoxc8tm1Phb/Hoxc8 +
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4K:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8+
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
4K
|
Expression was detected in motor neurons. The specimen was from the level of the cervical vertebra 8.
|
Specimen
4L:
embryonic day 14.5; Hoxc8tm1Phb/ Hoxc8tm1Phb
(more )
Note: Double labeled: red - Isl1 and Isl2; green - TUNEL.
Specimen
4L:
(close )
Genetic Background:
|
either: (involves: 129) or (involves: 129S2/SvPas * C57BL/6 * DBA/2)
|
Mutant Allele(s):
|
Hoxc8tm1Phb/Hoxc8tm1Phb
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Isl1 and Isl2; green - TUNEL.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Present |
Single cells |
4L
|
Expression was detected in motor neurons, but there were fewer motor neurons in the mutant. The specimen was from the level of the cervical vertebra 8.
|
|