Probe:	Nelf probe1
Probe preparation:	Antisense labelled with S35 DNA
Assay notes:	The probe used in this assay was 3' labelled.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong		1A
TS22: olfactory epithelium	Strong		1A
TS22: vomeronasal organ	Strong		1A
TS22: telencephalon	Strong	Regionally restricted	1A	Strong expression was detected in the hippocampus and cerebral cortex.
TS22: dorsal root ganglion	Present		1A
TS22: olfactory lobe	Present		1A
TS22: spinal cord	Present		1A
TS22: thalamus	Present		1A
TS22: trigeminal ganglion	Present		1A

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Strong		1B

Structure	Level	Pattern	Image	Note
TS22: olfactory lobe	Strong		1C	Strong expression was detected in the olfactory bulb.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Regionally restricted	1D	The expression detected in the developing hippocampus and cerebral cortex extended from the ventricular layer to the apical layer, with the highest level in cells closer to the apical surface.
TS22: telencephalon	Strong	Regionally restricted	1D	The expression detected in the developing hippocampus and cerebral cortex extended from the ventricular layer to the apical layer, with the highest level in cells closer to the apical surface.

Structure	Level	Pattern	Image	Note
TS22: thalamus	Strong	Uniform	1E	Expression was spread throughout the the developing subnuclei.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2B
TS28: hippocampus	Present		2B
TS28: main olfactory bulb	Present		2B
TS28: thalamus	Present		2B
TS28: cerebellum	Weak		2B
TS28: spinal cord	Absent		2B