Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:51992
Tsuda T, et al., Differential expression of flectin in the extracellular matrix and left-right asymmetry in mouse embryonic heart during looping stages. Dev Genet. 1998;23(3):203-14
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3713833
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Gene symbol:
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Myh9
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Gene name:
|
myosin, heavy polypeptide 9, non-muscle
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Antibody:
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mAb F22
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Detection system:
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Secondary antibody coupled to Cy3
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Assay notes:
|
The antibody recognizes two proteins; both have been annotated.
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|
Results
|
Specimen
Not shown:
embryonic day 8.0
(more )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: cardiac muscle tissue |
Present |
Regionally restricted |
|
Expression is in the myocardial extracellular matrix.
|
Specimen
1A:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
1B:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
1C:
embryonic day 8.5
(more )
Note: Embryos were embedded in araldite.
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: cardiac jelly |
Present |
Regionally restricted |
1C
|
Expression is in the extracellular matrix.
|
TS13: cardiac muscle tissue |
Present |
Regionally restricted |
1C
|
Expression is in the extracellular matrix in the myocardium.
|
Specimen
3A:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.75
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
3B:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.75
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
3C:
embryonic day 8.75
(more )
Note: Embryos were embedded in araldite.
Specimen
3C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 8.75
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
4A,B:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
4A,B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: heart |
Strong |
Regionally restricted |
4A
,
4B
|
Expression is in the inner curvature of the looping heart and on the left side of the distal tubular heart.
|
TS14: cardiac muscle tissue |
Present |
Regionally restricted |
4A
,
4B
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Expression is in the extracellular matrix of the myocardium.
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TS14: cardiac jelly |
Present |
|
4B
,
4A
|
|
Specimen
Not shown:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: outflow tract |
Present |
Regionally restricted |
|
There is increased right-sided expression in the outflow tract.
|
Specimen
5:
embryonic day 9.0
(more )
Note: Embryos were embedded in araldite.
Specimen
5:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
6A:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: primitive ventricle |
Present |
Regionally restricted |
6A
|
Expression is localized at the left side of common ventricle.
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TS15: outflow tract |
Present |
Graded |
6A
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There is increased flectin expression on the right side of the outflow tract.
|
TS15: endocardial lining |
Present |
Regionally restricted |
6A
|
Expression is in the lining of the endocardial endothelium.
|
Specimen
6B:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: primitive ventricle |
Present |
Regionally restricted |
6B
|
Expression is localized at the left side of common ventricle.
|
Specimen
6C:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
6C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: primitive ventricle |
Present |
Regionally restricted |
6C
|
Expression is localized at the left side of common ventricle.
|
Specimen
Not shown:
embryonic day 9.5
(more )
Note: Embryos were embedded in araldite.
Specimen
Not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Specimen
7:
embryonic day 11.5
(more )
Note: Embryos were embedded in araldite.
Specimen
7:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 11.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: visceral pericardium |
Present |
Regionally restricted |
7
|
Expression is in the epicardium of the right ventricular wall and atrial wall.
|
TS19: heart |
Weak |
Regionally restricted |
7
|
Flectin is also mildly expressed in the trabeculated myocardial extracellular matrix in the right ventricular sinus.
|
Specimen
8A:
embryonic day 9.5; Invsinv/ Invsinv
(more )
Note: Embryos were embedded in araldite.
Specimen
8A:
(close )
Genetic Background:
|
involves: FVB/N
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: outflow tract |
Present |
Regionally restricted |
8A
|
Expression is predominantly in the left side of outflow tract, which is the mirror image of the normal flectin expression pattern.
|
Specimen
8B:
embryonic day 9.5; Invsinv/ Invsinv
(more )
Note: Embryos were embedded in araldite.
Specimen
8B:
(close )
Genetic Background:
|
involves: FVB/N
|
Mutant Allele(s):
|
Invsinv/Invsinv
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Resin/plastic
|
Note:
|
Embryos were embedded in araldite.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: heart |
Present |
|
8B
|
Expression is the right side of ventricular tubular heart.
|
TS15: cardiac jelly |
Present |
|
8B
|
Expression in the cardiac jelly is quite similar to that of normal mouse embryos.
|
|