Thratm1Ffla
Targeted Allele Detail
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| Symbol: |
Thratm1Ffla |
| Name: |
thyroid hormone receptor alpha; targeted mutation 1, Frederic Flamant |
| MGI ID: |
MGI:3758914 |
| Synonyms: |
TRalpha1L400R, TRalphaAMI |
| Gene: |
Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
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| Alliance: |
Thratm1Ffla page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:125353
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129/Sv
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| Allele Type: |
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Targeted (Conditional ready, Dominant negative) |
| Mutation: |
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Nucleotide substitutions
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Mutation details: The open reading frame was replaced with a construct containing a 5' floxed neo cassette, a cDNA encoding the Thra open reading frame with a nucleotide substitution that results in an amino acid substitution of arginine for leucine at position 400 (L400R), and a 3' IRES-Tau-lacZ cassette. The L400R mutation is thought to place the receptor in an unliganded state and create a dominant negative receptor. Expression of the mutant receptor and lacZ is only achieved by cre-mediated removal of the floxed neo cassette. Western blot analysis was used to confirm expression of the mutant receptor following cre-mediated recombination.
(J:125353)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Thra Mutation: |
40 strains or lines available
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| Original: |
J:125353 Quignodon L, et al., A Point Mutation in the Activation Function 2 Domain of Thyroid Hormone Receptor {alpha}1 Expressed after CRE-Mediated Recombination Partially Recapitulates Hypothyroidism. Mol Endocrinol. 2007 Oct;21(10):2350-2360 |
| All: |
19 reference(s) |
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Analysis Tools
