Antibody:	EEDA antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	5C	Expression is in peripheral epithelia. Expression is in the basal and wing cells, but not in the superficial cells. Little, if any expression can be detected in the limbal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	5D	Expression is in central corneal epithelia.

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present	Regionally restricted	6D	Within the follicular epithelium expression is detected in the cells immediately above the line of Auber.

Structure	Level	Pattern	Image	Note
TS28: integumental system	Present	Regionally restricted	6E	Expression is in the intermediate cells of the nail matrix but not in the cells of the nail plate.

Structure	Level	Pattern	Image	Note
TS28: tongue epithelium	Present	Regionally restricted	6F	Expression is in the intermediate cells of the posterior portion of the dorsal tongue epithelium and is absent in the anterior portion.

Structure	Level	Pattern	Image	Note
TS28: epidermis suprabasal layer	Strong		7C
TS28: epidermis granular layer	Trace		7C

Structure	Level	Pattern	Image	Note
TS28: epidermis	Strong	Regionally restricted	7G	Expression is in the suprabasal cells immediately above the basal layer in the footpad epithelium. Little if expression is in the granular cells.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Trace	Regionally restricted	8A	Expression is in the outer cells of the two cell layered ectoderm.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	8B	Expression is in the cells immediately above the basal layer, but is absent in basal cells and follicular buds.

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present	Regionally restricted	8C	Expresion is above the basal layer and can not be detected in the prominent granular layer.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Strong	Regionally restricted	8D	Expression is in the suprabasal cells and little if any staining the granular or horny cells.

Structure	Level	Pattern	Image	Note
TS27: cornea epithelium	Present	Regionally restricted	9A	Expression is in the limbal, peripheral, and central corneal epithelia.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9B	Expression is absent in the limbal epithelium, faint presence in the peripheral corneal epithelium and distinct expression in the central corneal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9C	Expression is absent in the limbal epithelium, faint presence in the peripheral corneal epithelium and distinct expression in the central corneal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9D	Expression is in the basal and wing cells, but not in the superficial cells of the corneal epithelium. Also in the peripheral corneal epithelium, but not in the limbal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9E	Expression is in the basal and wing cells, but not in the superficial cells of the corneal epithelium. Also in the peripheral corneal epithelium, but not in the limbal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9F	Expression is in the basal and wing cells, but not in the superficial cells of the corneal epithelium. Also in the peripheral corneal epithelium, but not in the limbal epithelium.

Structure	Level	Pattern	Image	Note
TS28: cornea epithelium	Present	Regionally restricted	9G	Expression is in the basal and wing cells, but not in the superficial cells of the corneal epithelium. Also in the peripheral corneal epithelium, but not in the limbal epithelium.

Structure	Level	Pattern	Image	Note
TS28: lens epithelium	Strong	Regionally restricted		Expression is at the equatorial zone, and is absent in the central epithelial cells.
TS28: ciliary epithelium	Present
TS28: retina epithelium	Absent