Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Lgr5tm1(cre/ERT2)Cle
Name:	leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1, Hans Clevers
MGI ID:	MGI:3764660
Synonyms:	Lgr5-cre, Lgr5CREER, Lgr5-creERT, Lgr5-CreERT2-IRES-EGFP, Lgr5-EGFP, Lgr5 EGFP-IRES-creERT2, Lgr5-EGFP-IRES-creERT2, Lgr5tm1(cre/ESR1)Cle, Lgr5UT
Gene:	Lgr5  Location: Chr10:115286219-115423685 bp, - strand  Genetic Position: Chr10, 63.51 cM
Alliance:	Lgr5tm1(cre/ERT2)Cle page

Germline Transmission:	Earliest citation of germline transmission: J:127123
Parent Cell Line:	IB10/E14IB10 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Inducible, Null/knockout, Recombinase, Reporter) Inducer:    tamoxifen Mutation:    Insertion   Lgr5tm1(cre/ERT2)Cle expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Lgr5 (MGI:1341817)   Mutation details: An EGFP-IRES-creERT2 cassette was inserted into exon 1 of the gene along with a loxP-flanked neomycin cassette. The neomycin gene was then excised in vivo by crossing with cre-deleter strain. GFP expression is driven by the endogenous promoter. Cre expression is induced in Lgr5 expressing cells by tamoxifen treatment. (J:127123)

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	40 assay results 9 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

List all tumor models in MMHCdb carrying Lgr5tm1(cre/ERT2)Cle

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Lgr5 Mutation:	58 strains or lines available

Original:	J:127123 Barker N, et al., Identification of stem cells in small intestine and colon by marker gene Lgr5. Nature. 2007 Oct 25;449(7165):1003-7
All:	558 reference(s)