Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:101025
Yaylaoglu MB, et al., Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform. Dev Dyn. 2005 Oct;234(2):371-86
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3766997
|
Gene symbol:
|
Trhde
|
Gene name:
|
TRH-degrading enzyme
|
Probe:
|
Trhde cDNA1
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
Assay notes:
|
A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
|
|
Results
|
Specimen
2D:
postnatal adult
(more )
Specimen
2D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus region |
Strong |
Uniform |
2D
|
There is a strong signal in all cells of the cornu ammonis.
|
TS28: dentate gyrus |
Absent |
|
2D
|
The signal in the dentate gyrus is similar to that seen in null mutants.
|
Specimen
2E:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Trhdetm1Lars/Trhdetm1Lars
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hippocampus |
Absent |
|
2E
|
The authors interpret the diffuse staining seen as nonspecific background staining.
|
Specimen
2F:
postnatal adult
(more )
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Strong |
Regionally restricted |
2F
|
Expression is present in individual neurons in the retrosplenial granular cortex.
|
Specimen
2G:
(close )
Genetic Background:
|
involves: 129P2/OlaHsd
|
Mutant Allele(s):
|
Trhdetm1Lars/Trhdetm1Lars
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
|