Shhtm6Amc
Targeted Allele Detail
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| Symbol: |
Shhtm6Amc |
| Name: |
sonic hedgehog; targeted mutation 6, Andrew P McMahon |
| MGI ID: |
MGI:3775846 |
| Synonyms: |
Shh::GFP |
| Gene: |
Shh Location: Chr5:28661838-28672099 bp, - strand Genetic Position: Chr5, 14.39 cM
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| Alliance: |
Shhtm6Amc page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:132152
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
129X1/SvJ
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| Allele Type: |
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Targeted (Null/knockout, Reporter) |
| Mutation: |
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Insertion
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Mutation details: A GFP cassette was inserted into exon 3 and an FRT site flanked neomycin resistance gene cassette was inserted between exon 2 and 3. The presence of the fusion protein and the absence of the endogenous product was confirmed by western blot analysis on E9.5 embryo extracts. This allele produces functional albeit inefficient protein product.
(J:132152)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Original: |
J:132152 Chamberlain CE, et al., Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development. 2008 Mar;135(6):1097-106 |
| All: |
7 reference(s) |
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Analysis Tools
