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Nefltm1(cre/ERT)Nat
Targeted Allele Detail
Summary
Symbol: Nefltm1(cre/ERT)Nat
Name: neurofilament, light polypeptide; targeted mutation 1, Jeremy Nathans
MGI ID: MGI:3777624
Synonyms: Nefltm1(cre/Esr1)Nat, NFL-IRES-creER, NFL-IRES-creER(T)
Gene: Nefl  Location: Chr14:68321312-68326544 bp, + strand  Genetic Position: Chr14, 35.46 cM, cytoband D3
Alliance: Nefltm1(cre/ERT)Nat page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:144315
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Nefltm1(cre/ERT)Nat expression driven by 1 gene
 
Mutation details: An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in ganglion cells and horizontal cells of the retina, and in a sparse number of neurons in the brain. (J:144315)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Nefl (mouse)
Summary of all recombinase alleles driven by Nefl.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nefl Mutation:  17 strains or lines available
Notes
ES cell line = JHMI-ES1.
References
Original:  J:144315 Rotolo T, et al., Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology. PLoS One. 2008;3(12):e4099
All:  5 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory