Isl1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:130227 Millien G, et al., Characterization of the mid-foregut transcriptome identifies genes regulated during lung bud induction. Gene Expr Patterns. 2008 Jan;8(2):124-39
Assay type:	RT-PCR
MGI Accession ID:	MGI:3784459
Gene symbol:	Isl1
Gene name:	ISL1 transcription factor, LIM/homeodomain

Probe:	Isl1-pE, Isl1-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Real-time RT-PCR assay.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
8.5	E8.5 (a)	TS13: foregut diverticulum	Present	Not Specified	CD-1		Not Specified	lung
9.5	E9.5 (a)	TS15: lung rudiment	Present	Not Specified	CD-1		Not Specified
11	E11.0 (a)	TS18: lung rudiment	Present	Not Specified	CD-1		Not Specified
13	E13.0 (a)	TS21: lung	Present	Not Specified	CD-1		Not Specified
15	E15.0 (a)	TS23: lung	Present	Not Specified	CD-1		Not Specified
16	E16.0 (a)	TS24: lung	Present	Not Specified	CD-1		Not Specified
17	E17.0 (a)	TS25: lung	Present	Not Specified	CD-1		Not Specified
18	E18.0 (a)	TS26: lung	Present	Not Specified	CD-1		Not Specified
19	E19.0 (a)	TS26: lung	Present	Not Specified	CD-1		Not Specified
Adult	P adult	TS28: lung	Present	Not Specified	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression is transient, the mRNA level peaks on embryonic day E9.5 and decreases at E11.5 to that of E8.5. Then there is a gradual decline during later development to levels that are about 10-15% of the peak value.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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