Assay
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Reference:
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J:132131
Barton KM, et al., Expression patterns and cell cycle profiles of PCNA, MCM6, cyclin D1, cyclin A2, cyclin B1, and phosphorylated histone H3 in the developing mouse retina. Dev Dyn. 2008 Mar;237(3):672-82
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3784865
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Gene symbol:
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Mcm6
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Gene name:
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minichromosome maintenance complex component 6
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Antibody:
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MCM6 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Tissue
samples were fixed in 4% neutral
buffered formalin.
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Results
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Specimen
Not shown:
embryonic day 9.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
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Age:
|
embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS15: optic cup |
Present |
Regionally restricted |
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Expression is in most cells of the retina and surrounding tissues.
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Specimen
1A,C:
embryonic day 11.5
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1A,C:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
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Age:
|
embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: lens vesicle |
Present |
|
1A
,
1C
|
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TS19: pigmented retinal epithelium |
Present |
|
1A
,
1C
|
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TS19: blood vessel |
Present |
Regionally restricted |
1C
,
1A
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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TS19: eye |
Present |
Regionally restricted |
1C
,
1A
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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TS19: head |
Present |
Regionally restricted |
1C
,
1A
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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Specimen
1D,F:
embryonic day 13.5
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1D,F:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
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Age:
|
embryonic day 13.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pigmented retinal epithelium |
Present |
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1D
,
1F
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TS21: retina |
Present |
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1D
,
1F
|
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TS21: blood vessel |
Present |
Regionally restricted |
1D
,
1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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TS21: eye |
Present |
Regionally restricted |
1D
,
1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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TS21: head |
Present |
Regionally restricted |
1D
,
1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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Specimen
1G,I:
embryonic day 16.5
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1G,I:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Formalin
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Embedding:
|
Cryosection
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Note:
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Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: retina nuclear layer |
Present |
Regionally restricted |
1I
,
1G
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Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.
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Specimen
1J,L:
postnatal day 3
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1J,L:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
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Age:
|
postnatal day 3
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
|
Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
1L
,
1J
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Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.
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Specimen
1M,O:
postnatal day 6
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1M,O:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina layer |
Present |
Regionally restricted |
1O
,
1M
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Expression is in a narrow band of cells in the developing inner nuclear layer.
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Specimen
1P,R:
postnatal day 15
(more )
Note: Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
Specimen
1P,R:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
|
Double labeled: green - Mcm6; red - Pcna (assay MGI:3784955).
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Specimen
4A,C:
embryonic day 13.5
(more )
Note: Double labeled: green - Mcm6; red - Tubb3 (assay MGI:3784956).
Specimen
4A,C:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Tubb3 (assay MGI:3784956).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: eye |
Present |
|
4C
,
4A
|
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TS21: retina |
Weak |
Regionally restricted |
4A
,
4C
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Weak expression in the inner retina.
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Specimen
4D,F:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Tubb3 (assay MGI:3784956).
Specimen
4D,F:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Tubb3 (assay MGI:3784956).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
4F
,
4D
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Expression is in the neuroblast layer in newly postmitotic cells.
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Specimen
4G,I:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Nefm (assay MGI:3784957).
Specimen
4G,I:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Nefm (assay MGI:3784957).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
4G
,
4I
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Expression is in the neuroblast layer in newly postmitotic cells.
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Specimen
4J,L:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Rcvrn (assay MGI:3784958).
Specimen
4J,L:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Mcm6; red - Rcvrn (assay MGI:3784958).
|
Specimen
4M,O:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Calb2 (assay MGI:3784959).
Specimen
4M,O:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Calb2 (assay MGI:3784959).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
4M
,
4O
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Expression is in the neuroblast layer in newly postmitotic cells.
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Specimen
7A,C:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Ccnd1 (assay MGI:3784960).
Specimen
7A,C:
(close )
Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Ccnd1 (assay MGI:3784960).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
7C
,
7A
|
Expression is in the neuroblast layer.
|
Specimen
7D,F:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Ccna2 (assay MGI:3784961).
Specimen
7D,F:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
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Age:
|
postnatal day 0
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Ccna2 (assay MGI:3784961).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
7D
,
7F
|
Expression is in the neuroblast layer.
|
Specimen
7G,I:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - BrdU.
Specimen
7G,I:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - BrdU.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
7G
,
7I
|
Expression is in the neuroblast layer.
|
Specimen
7J,L:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - Ccnb1 (assay MGI:3784962).
Specimen
7J,L:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - Ccnb1 (assay MGI:3784962).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
7L
,
7J
|
Expression is in the neuroblast layer.
|
Specimen
7M,O:
postnatal day 0
(more )
Note: Double labeled: green - Mcm6; red - pHH3.
Specimen
7M,O:
(close )
Genetic Background:
|
either: Black Swiss or Swiss Webster
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Mcm6; red - pHH3.
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina layer |
Present |
Regionally restricted |
7M
,
7O
|
Expression is in the neuroblast layer.
|
|