Hesx1tm3Jpmb
Targeted Allele Detail
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| Symbol: |
Hesx1tm3Jpmb |
| Name: |
homeobox gene expressed in ES cells; targeted mutation 3, Juan Pedro Martinez Barbera |
| MGI ID: |
MGI:3822475 |
| Synonyms: |
Hesx1R160C |
| Gene: |
Hesx1 Location: Chr14:26716373-26724286 bp, + strand Genetic Position: Chr14, 16.09 cM, cytoband A3-B
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| Alliance: |
Hesx1tm3Jpmb page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:142649
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted |
| Mutation: |
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Nucleotide substitutions
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Mutation details: The coding sequence for wild-type Hesx1 was mutated by PCR so that codon 160 (arginine) was changed to encode a cysteine residue (CGA->TGC). A loxP-flanked PGK-Neo selection cassette was inserted into the first intron of the mutated Hesx1 sequence in the targeting vector. The orientation of the PGK-Neo was inverted relative to the normal direction of transcription in an attempt to reduce expression of the mutated allele, which may have a dominant negative effect in ES cells where the Hesx1 locus is transcripitionally active. Homologous recombination in CCE ES cells replaced the wild-type sequence with the mutant sequence in the targeting vector. F1 animals were crossed with Actin-beta-cre mice to excise the PGK-Neo cassette.
(J:142649)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Hesx1 Mutation: |
14 strains or lines available
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| Original: |
J:142649 Sajedi E, et al., Analysis of mouse models carrying the I26T and R160C substitutions in the transcriptional repressor HESX1 as models for septo-optic dysplasia and hypopituitarism. Dis Model Mech. 2008 Nov;1(4-5):241-54 |
| All: |
1 reference(s) |
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Analysis Tools
