Apc<tm1Tyj> Targeted Allele Detail MGI Mouse (MGI:3829069)

Apc^tm1Tyj
Targeted Allele Detail

Summary

Symbol:	Apc^tm1Tyj
Name:	APC, WNT signaling pathway regulator; targeted mutation 1, Tyler Jacks
MGI ID:	MGI:3829069
Synonyms:	APC^fle1-15
Gene:	Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
Alliance:	Apc^tm1Tyj page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:159014
Parent Cell Line:	V26.2 (ES Cell)
Strain of Origin:	C57BL/6

Mutation
description

Allele Type:		Targeted (Conditional ready, No functional change)
Mutation:		Insertion
		Mutation details: A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. (J:159014)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

Tumor Data

List all tumor models in MMHCdb carrying Apc^tm1Tyj

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Apc Mutation:	158 strains or lines available

References

Original:	J:159014 Jacks T, Generation of a conditional Apc allele. MGI Direct Data Submission. 2010;
All:	33 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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