Summary | Mutation origin | Mutation description | Expression | Recombinase activity | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Cpa3tm3(icre)Hrr
Name:	carboxypeptidase A3, mast cell; targeted mutation 3, Hans-Reimer Rodewald
MGI ID:	MGI:3829878
Synonyms:	Cpa3Cre, Cpa3tm3Hrr
Gene:	Cpa3  Location: Chr3:20269784-20296345 bp, - strand  Genetic Position: Chr3, 6.25 cM
Alliance:	Cpa3tm3(icre)Hrr page

Germline Transmission:	Earliest citation of germline transmission: J:143731
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Recombinase) Mutations:    Insertion, Intragenic deletion   Cpa3tm3(icre)Hrr expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Cpa3 (MGI:88479)   Mutation details: The coding sequence for the first 28 nucleotides of the gene locus was replaced with a codon-improved cre recombinase expression cassette and a Frt-flanked neomycin selection cassette. Transient expression of Flp-recombinase in correctly targeted ES cells removed the selection cassette. When mice carrying this cre knock-in allele were crossed with mice carrying the cre-dependent reporter allele Gt(ROSA)26Sortm1Hjf, reporter expression was observed in about 25% of early pro-T cells in the thymus. By the DN2 stage (CD44+CD25+CD4-CD8-) of T cell development, 90% of the cells expressed the reporter as did 90% of peripheral T cells. A small percentage (<5%) of mature B cells also expressed the reporter. Bone marrow analysis demonstrated expression in some (10-30%) stem cells, common lymphoid progenitors and in myeloid- and plasmacytoid- dendritic cells. (J:143731, J:178942)

Activity:	Tissue activity of this recombinase allele
Driver:	Cpa3 (mouse) Summary of all recombinase alleles driven by Cpa3. Alliance mouse Cpa3 gene page

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

List all tumor models in MMHCdb carrying Cpa3tm3(icre)Hrr

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cpa3 Mutation:	30 strains or lines available

Original:	J:143731 Feyerabend TB, et al., Deletion of Notch1 converts pro-T cells to dendritic cells and promotes thymic B cells by cell-extrinsic and cell-intrinsic mechanisms. Immunity. 2009 Jan;30(1):67-79
All:	27 reference(s)