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Hprt1tm10(Ple162-EGFP/cre)Ems
Targeted Allele Detail
Summary
Symbol: Hprt1tm10(Ple162-EGFP/cre)Ems
Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 10, Elizabeth M Simpson
MGI ID: MGI:3835521
Synonyms: Hprt1tm10(mEMS954)Ems, Hprt1tm10(Ple162-EGFPcre;mEMS954)Ems, PITX3-EGFP/cre
Gene: Hprt1  Location: ChrX:52077014-52110536 bp, + strand  Genetic Position: ChrX, 29.31 cM, cytoband A6
Alliance: Hprt1tm10(Ple162-EGFP/cre)Ems page
Mutation
origin
Mutant Cell Lines:  mEMS951, mEMS953, mEMS954
Germline Transmission:  Earliest citation of germline transmission: J:145689
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Pleiades Promoter Project
Mutation
description
Allele Type:    Targeted (Recombinase, Reporter)
Mutation:    Insertion     Vector Type: Not Applicable
 
Hprt1tm10(Ple162-EGFP/cre)Ems expression driven by 1 gene
 
Mutation details: The Ple162-EGFPCre transgene (pEMS1148) was designed with the 3636 bp Ple162 minipromoter (PITX3-D; derived from an intronic candidate regulatory region and a segment of the human paired-like homeodomain 3 (PITX3) promoter) upstream of a minimal F5 mutant-frt site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). (J:164356)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: PITX3 (human)
Summary of all recombinase alleles driven by PITX3.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hprt1 Mutation:  1279 strains or lines available
Notes
Germ line transmission of mutant cell line mEMS954 has been established.
References
Original:  J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009;
All:  2 reference(s)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory